2026-06-29T21:03:14Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/55772021-11-02T12:00:41Zcom_10259_5827com_10259_5086com_10259_2604col_10259_5828

Gerardi, Gisela Casali, Cecilia I. Cavia Saiz, Mónica Rivero Pérez, Maria Dolores Perazzo, Cecilia González San José, Mª Luisa Muñiz Rodríguez, Pilar Fernández Tome, María C. 2020-12-11T08:37:22Z 2020-12-11T08:37:22Z 2020-11 2405-8440 http://hdl.handle.net/10259/5577 10.1016/j.heliyon.2020.e05396 The functional renal epithelium is composed of differentiated and polarized tubular cells with a strong actin cortex and specialized cell-cell junctions. If, under pathological conditions, these cells have to resist higher kidney osmolarity, they need to activate diverse mechanisms to survive external nephrotoxic agents such as inflammation and oxidative stress. Wine pomace polyphenols exert protective effects on renal cells. In this study, two wine-pomace products and their protective effects upon promotion and preservation of normal cell differentiation and attenuation of oxalate-induced type II epithelial mesenchymal transition (EMT) are evaluated. Treatment with gastrointestinal and colonic bioavailable fractions from red (rWPP) and white (wWPP) wine pomaces, both in the presence and the absence of oxalate, showed similar cell numbers and nuclear size than the non-treated differentiated MDCK cells. Immunofluorescence analysis showed the reduction of morphological changes and the preservation of cellular junctions for the rWPP and wWPP pre-treatment of cells exposed to oxalate injury. Hence, both rWPP and wWPP attenuated oxalate type II EMT in MDCK cells that conserved their epithelial morphology and cellular junctions through the antioxidant activities of grape pomace polyphenols. Agencia Nacional de Promoci on Científica y Tecnol ogica (PICT 2016-1055), the University of Buenos Aires (UBACYT 2014-2017, 20020130100658BA) and the Ministerio de Ciencia, Innovaci on y Universidades (PGC2018-097113B100). application/pdf eng Elsevier Heliyon. 2020, V. 6, n. 11, e05396 https://doi.org/10.1016/j.heliyon.2020.e05396 info:eu-repo/grantAgreement/MICINN/PGC2018-097113B100 info:eu-repo/grantAgreement/UBA/UBACYT-2014-2017-20020130100658B info:eu-repo/grantAgreement/Agencia Nacional de Promoción Científica y Tecnológica/PICT 2016-1055 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Food science Cell biology Plant products Cell differentiation Cytoskeleton Natural product Polyphenol Antioxidant Cancer research Renal system Wine pomace Polyphenols EMT Oxalate E-cadherin Alimentos Biología molecular Bioquímica Food Molecular biology Biochemistry Bioavailable wine pomace attenuates oxalate-induced type II epithelial mesenchymal transition and preserve the differentiated phenotype of renal MDCK cells info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Heliyon 6 11 e05396