2026-04-28T08:55:03Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/55892024-07-25T07:18:06Zcom_10259_9476com_10259.4_106com_10259_2604col_10259_9477

Odoni, Dorett I 2f71946a-bfcc-41d3-9895-801dd6e803f0 500 Vazquez Vilar, Marta 5e73186f-073f-420a-8bbc-1192e3d7a3dc 500 Gaal, Merlijn P van 189b0938-6a95-4ab5-befa-c5fc563f3fdd 500 Schonewille, Tom e6b46aae-d203-444b-a5ae-12dc07fed952 500 Santos, Vitor AP Martins dos eb46c818-2482-4f90-ab35-57f7bc8197eb 500 Tamayo Ramos, Juan Antonio 547 500 0000-0002-7071-002X Suarez Diez, Maria 02e00910-18d5-46be-b42a-e43cca9f9803 500 Schaap, Peter J. f6d7edbb-1902-4228-b6fc-d769a702a41a 500 2021-01-12T11:53:43Z 2021-01-12T11:53:43Z 2019-04 0378-1097 http://hdl.handle.net/10259/5589 10.1093/femsle/fnz071 1574-6968 Currently, there is no consensus regarding the mechanism underlying Aspergillus niger citrate biosynthesis and secretion. We hypothesise that depending on the experimental setup, extracellular citrate accumulation can have fundamentally different underlying transcriptomic landscapes. We show that varying the amount and type of supplement of an arginine auxotrophic A. niger strain results in transcriptional down-regulation of citrate metabolising enzymes in the condition in which more citrate is accumulated extracellularly. This contrasts with the transcriptional adaptations when increased citrate production is triggered by iron limitation. By combining gene expression data obtained from these two very distinct experimental setups with hidden Markov models and transporter homology approaches, we were able to compile a shortlist of the most likely citrate transporter candidates. Two candidates (An17g01710 and An09g06720m.01) were heterologously expressed in the yeast Saccharomyces cerevisiae, and one of the resultant mutants showed the ability to secrete citrate. Our findings provide steps in untangling the complex interplay of different mechanisms underlying A. niger citrate accumulation, and we demonstrate how a comparative transcriptomics approach complemented with further bioinformatics analyses can be used to pinpoint a fungal citrate exporter. grant in the framework of the BE-BASIC program F01.011 Transport processes in the production of organic acids by Aspergillus niger, and the WUR IPOP Systems Biology program KB-17-003.02.026 Genome-wide metabolic modelling and data integration of organic acid production in filamentous fungi. application/pdf eng Oxford University Press FEMS Microbiology. 2021, V. 366 ,n. 7, fnz071 https://doi.org/10.1093/femsle/fnz071 Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Aspergillus niger citrate transport transcriptomics homology MDR Microbiología Microbiology Aspergillus niger citrate exporter revealed by comparison of two alternative citrate producing conditions info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion FEMS Microbiology Letters TEXT Odini-fml_2019.pdf.txt Odini-fml_2019.pdf.txt Extracted text text/plain 55449 https://riubu.ubu.es/bitstream/10259/5589/5/Odini-fml_2019.pdf.txt dbc508172ebcfb6f32c5851f07beafa2 MD5 5 THUMBNAIL Odini-fml_2019.pdf.jpg Odini-fml_2019.pdf.jpg IM Thumbnail image/jpeg 4999 https://riubu.ubu.es/bitstream/10259/5589/4/Odini-fml_2019.pdf.jpg ac7769a6777ca09a2d046237dc0b6701 MD5 4 LICENSE license.txt license.txt text/plain; charset=utf-8 998 https://riubu.ubu.es/bitstream/10259/5589/3/license.txt aa90105e1c90a1b4fe810649aa967b7d MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 908 https://riubu.ubu.es/bitstream/10259/5589/2/license_rdf 0175ea4a2d4caec4bbcc37e300941108 MD5 2 ORIGINAL Odini-fml_2019.pdf Odini-fml_2019.pdf application/pdf 634775 https://riubu.ubu.es/bitstream/10259/5589/1/Odini-fml_2019.pdf 354d071af1ee1d2f405da32911d61ee6 MD5 1 10259/5589 oai:riubu.ubu.es:10259/5589 2024-07-25 09:18:06.1 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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