2026-05-06T10:00:16Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/57782024-07-25T07:26:03Zcom_10259_9476com_10259.4_106com_10259_2604col_10259_9477

Laothanachareon, Thanaporm f9e6706b-1ef7-4cca-8754-7963eb56a5c5 500 Bruinsma, Lyon 7699ae9b-2837-4b2c-87e0-36686d664773 500 Nijsse, Bart a32fe2cd-7aa9-49f8-a3c2-d1f2ec05044f 500 Schonewille, Tom e6b46aae-d203-444b-a5ae-12dc07fed952 500 Suarez Diez, Maria 02e00910-18d5-46be-b42a-e43cca9f9803 500 Tamayo Ramos, Juan Antonio 547 500 0000-0002-7071-002X Santos, Vitor AP Martins dos eb46c818-2482-4f90-ab35-57f7bc8197eb 500 Schaap, Peter J. f6d7edbb-1902-4228-b6fc-d769a702a41a 500 2021-05-24T07:58:51Z 2021-05-24T07:58:51Z 2021-06 http://hdl.handle.net/10259/5778 10.3390/jof7060409 2309-608X Aspergillus niger is the major industrial citrate producer worldwide. Export as well as uptake of citric acid are believed to occur by active, proton-dependent, symport systems. Both are major bottlenecks for industrial citrate production. Therefore, we assessed the consequences of deleting the citT gene encoding the A. niger citrate exporter, effectively blocking active citrate export. We followed the consumption of glucose and citrate as carbon sources, monitored the secretion of organic acids and carried out a thorough transcriptome pathway enrichment analysis. Under controlled cultivation conditions that normally promote citrate secretion, the knock-out strain secreted negligible amounts of citrate. Blocking active citrate export in this way led to a reduced glucose uptake and a reduced expression of high-affinity glucose transporter genes, mstG and mstH. The glyoxylate shunt was strongly activated and an increased expression of the OAH gene was observed, resulting in a more than two-fold higher concentration of oxalate in the medium. Deletion of citT did not affect citrate uptake suggesting that citrate export and citrate uptake are uncoupled from the system. application/pdf eng MDPI Journal of Fungi. 2021, V. 7, n. 6, 409. https://doi.org/10.3390/jof7060409 Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Aspergillus niger citrate exporter citT gene cexA gene transcriptome citric acid oxalic acid Microbiología Microbiology Global transcriptional response of aspergillus niger to blocked active citrate export through deletion of the exporter gene info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Journal of Fungi 7 6 409 THUMBNAIL Laothanachareon-jof_2021.pdf.jpg Laothanachareon-jof_2021.pdf.jpg IM Thumbnail image/jpeg 3519 https://riubu.ubu.es/bitstream/10259/5778/4/Laothanachareon-jof_2021.pdf.jpg 08ef9c8169cf17f65098dca17e97d892 MD5 4 LICENSE license.txt license.txt text/plain; charset=utf-8 998 https://riubu.ubu.es/bitstream/10259/5778/3/license.txt aa90105e1c90a1b4fe810649aa967b7d MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 908 https://riubu.ubu.es/bitstream/10259/5778/2/license_rdf 0175ea4a2d4caec4bbcc37e300941108 MD5 2 ORIGINAL Laothanachareon-jof_2021.pdf Laothanachareon-jof_2021.pdf application/pdf 1190742 https://riubu.ubu.es/bitstream/10259/5778/1/Laothanachareon-jof_2021.pdf c58971665d2cf7281934b33e6229e7d7 MD5 1 10259/5778 oai:riubu.ubu.es:10259/5778 2024-07-25 09:26:03.727 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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