2026-04-28T11:37:54Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/57842024-07-25T07:31:53Zcom_10259_9476com_10259.4_106com_10259_2604col_10259_9477

Schindler, Torben ce04c86e-b000-4745-8ed7-d4238acf010d 500 González, Adrián 97a95592-ee81-43ef-b323-21b5edf029b0 500 Boopathi, Ramachandran 44e92b2f-5594-4aa9-97be-77851c805799 500 Marty Roda, Marta 350 500 0000-0002-5440-1454 Romero Santacreu, Lorena 489 500 Wildes, Andew R. 76ed1e4f-3067-436d-b727-24a0da424131 500 Porcar, Lionel 68851b3f-bbb0-4ba2-8510-a8a109f1e946 500 Martel, Anne ccf1709a-b4b9-4323-bc48-3f873b26e9f2 500 Theodorakopoulos, Nikos 7d281b39-6295-40f6-bec5-21fa8db7ad9e 500 Cuesta López, Santiago 133 500 0000-0002-7401-3889 Angelov, Dimitar 5a0eaf3e-154d-4dba-b8ca-4205aabc667b 500 Unruh, Tobias 42360952-4acb-4b24-b70b-036b09ee14c3 500 Peyrard, Michel b48babc4-3814-46ff-b949-70919d2afe93 500 2021-05-25T10:06:10Z 2021-05-25T10:06:10Z 2018-10 2470-0045 http://hdl.handle.net/10259/5784 10.1103/PhysRevE.98.042417 2470-0053 DNA is a flexible molecule, but the degree of its flexibility is subject to debate. The commonly-accepted persistence length of lp ≈ 500Å is inconsistent with recent studies on short-chain DNA that show much greater flexibility but do not probe its origin. We have performed x-ray and neutron small-angle scattering on a short DNA sequence containing a strong nucleosome positioning element and analyzed the results using a modified Kratky-Porod model to determine possible conformations. Our results support a hypothesis from Crick and Klug in 1975 that some DNA sequences in solution can have sharp kinks, potentially resolving the discrepancy. Our conclusions are supported by measurements on a radiation-damaged sample, where single-strand breaks lead to increased flexibility and by an analysis of data from another sequence, which does not have kinks, but where our method can detect a locally enhanced flexibility due to an AT domain. Spanish Ministry of Economy, Industry and Competitiveness (BES-2013-065453, EEBB-I-2015-09973, FIS2012-38827). S.C.L. and UC-154 are grateful for the support of Junta de Castilla y Leon (Spain) Nanofibersafe BU079U16. D.A. acknowledges funding from the Agence Nationale de la Recherche through ANR-12-BSV5-0017-01 “Chrome” and ANR-17-CE11-0019-03 “Chrom3D” grants. N.T. acknowledges support by the project Advanced Materials and Devices (MIS 5002409, Competitiveness, Entrepreneurship and Innovation, NSRF 2014-2020) cofinanced by Greece and the European Regional Development Fund. application/pdf eng American Physical Society Physical Review E. 2018, V. 98, n. 4, 042417 https://doi.org/10.1103/PhysRevE.98.042417 info:eu-repo/grantAgreement/MINECO/BES-2013-065453 info:eu-repo/grantAgreement/MINECO/EEBB-I-2015-09973 info:eu-repo/grantAgreement/MINECO/FIS2012-38827 info:eu-repo/grantAgreement/JCyL/BU079U16 Física Physics Kinky DNA in solution: Small-angle-scattering study of a nucleosome positioning sequence info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion info:eu-repo/semantics/openAccess Physical Review E 98 4 THUMBNAIL Schindler-pre_2018.pdf.jpg Schindler-pre_2018.pdf.jpg IM Thumbnail image/jpeg 3396 https://riubu.ubu.es/bitstream/10259/5784/3/Schindler-pre_2018.pdf.jpg 94ecfbf864c510c389418afe1b3dc2c2 MD5 3 LICENSE license.txt license.txt text/plain; charset=utf-8 998 https://riubu.ubu.es/bitstream/10259/5784/2/license.txt aa90105e1c90a1b4fe810649aa967b7d MD5 2 ORIGINAL Schindler-pre_2018.pdf Schindler-pre_2018.pdf application/pdf 1787163 https://riubu.ubu.es/bitstream/10259/5784/1/Schindler-pre_2018.pdf a75c3b19d38660c2c0e5c5ee4266ae08 MD5 1 10259/5784 oai:riubu.ubu.es:10259/5784 2024-07-25 09:31:53.937 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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