2026-04-28T14:24:00Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/61242022-11-15T13:25:37Zcom_10259_3844com_10259_5086com_10259_2604col_10259_3845

Derivative UV/Vis spectroelectrochemistry in a thin-layer regime: deconvolution and simultaneous quantification of ascorbic acid, dopamine and uric acid Olmo Alonso, Fabiola Garoz Ruiz, Jesús Colina Santamaría, Álvaro Heras Vidaurre, Aránzazu Spectroelectrochemistry PARAFAC Quantitative analysis Derivative of absorbance with respect towavelength Química analítica Chemistry, Analytic In this work, UV/Vis spectroelectrochemistry (SEC), in a thin-layer regime and parallel configuration, is selected to solve a complex mixture that contains dopamine (DA), ascorbic acid (AA) and uric acid (UA). These molecules, like many other biological compounds, are assuming a highly important place in analytical and biomedical fields due to the fundamental role that they play in human metabolism. In addition, low or high levels of these compounds are associated with diseases such as Parkinson’s disease. For this reason, the quantification of these biomolecules is becoming increasingly critical. However, some drawbacks must be overcome, because the three molecules coexist in the human body, and the species are subject to mutual interference. In fact, they are all oxidized at similar potentials, and their UV/Vis absorption bands overlap, greatly complicating their quantification. For this reason, derivative SEC together with suitable chemometric tools such as PARAFAC are proposed to solve this complex matrix. This technique allows us to separate the contribution of each of these molecules present in a sample and to quantify all of them, achieving high resolution and reproducibility. Besides, detection limits at the micromolar level are achieved for DA, AA and UA in mixture solutions. This work thus demonstrates the great potential for derivative potentiodynamic SEC combined with the appropriate chemometric tools in solving complex mixtures, a field where SEC is still taking the first steps. Ministerio de Economía y Competitividad (Grants CTQ2017-83935-RAEI/ FEDER, UE), Junta de Castilla y León (Grant BU297P18) and Ministerio de Ciencia, Innovación y Universidades (RED2018-102412- T). F.O. is grateful for the contract funded by Junta de Castilla y León, the European Social Fund and the Youth Employment Initiative. J.G.R. thanks theMinisterio de Economía y Competitividad for his postdoctoral contract (CTQ2017-83935-R AEI/FEDER, UE). 2021-11-08T12:56:54Z 2021-11-08T12:56:54Z 2020-09 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion 1618-2642 http://hdl.handle.net/10259/6124 10.1007/s00216-020-02564-1 1618-2650 spa Analytical and Bioanalytical Chemistry. 2020, V. 412, n. 24, p. 6329–6339 https://doi.org/10.1007/s00216-020-02564-1 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2017-83935-R/ES/ESPECTROELECTROQUIMICA RAMAN CUANTITATIVA info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/ RED2018-102412-T/ES/RED DE SENSORES Y BIOSENSORES ELECTROQUIMICOS info:eu-repo/grantAgreement/Junta de Castilla y León//BU297P18 info:eu-repo/semantics/openAccess application/pdf Springer