2026-05-11T22:52:46Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/66572022-05-20T13:22:04Zcom_10259_4244com_10259_5086com_10259_2604col_10259_4569

Alonso Riaño, Patricia aad9756c-ad87-4f31-ba02-459668ce3469 600 Melgosa Gómez, Rodrigo 357 600 Trigueros Andrés, Ester 557 600 Benito Román, Oscar 59 600 0000-0002-4418-0045 Illera Gigante, Alba Ester 296 600 Beltrán Calvo, Sagrario 57 600 Sanz Díez, Mª Teresa 530 600 2022-05-16T09:14:39Z 2022-05-16T09:14:39Z 2022 http://hdl.handle.net/10259/6657 Póster presentado en: EIFS2022, the 2nd Iberian Meeting on Supercritical Fluids (2º Encontro Ibérico de Fluidos Supercríticos / 2º Encuentro Ibérico de Fluidos Supercríticos), to be held on 28.February - 2.March 2022 in Coimbra, Portugal. Brewer’s spent grain (BSG) is one of the most important by-products in large and small- scale breweries. BSG is the solid residue generated after mashing and wort filtration process, it is generated at an average rate of 20 kg per 100 L of beer and accounts for approximately 85 % of the total residues of the brewing process [4]. Nowadays, it is mainly used for animal feed (70 %), biogas production (10 %), or landfilled [5]. However, BSG presents a valuable chemical composition with a high content of protein and carbohydrates, as well as important quantities of phenolic compounds with potential bioactive properties. BSG also contains non-negligible amount of lipids (5 %) with more than 50 % being linoleic acid (C18:2 ω-6) [6]. Due to the valuable chemical composition of BSG, different techniques have been proposed to valorize this lignocellulosic biomass, such as enzymatic and chemical hydrolysis, ultrasound assisted extraction or microwave assisted extraction [7]. High pressure processing of biomass has been also proposed since it offers unique opportunities in the extraction and valorization of the bioactive compounds of BSG. Among the different high-pressure processes, the use of supercritical CO2 (sc-CO2) presents a great attractive since it is considered a green solvent and it presents gas-like (high diffusivities) and liquid-like (good solvation power) properties at supercritical conditions (Tc = 31.1 oC pc= 7.39 MPa). Sc-CO2 has been extensively studied as a green extracting agent over traditional organic solvents to valorize the lipophilic fraction of biomass [8]. From a biorefinery perspective and aiming at the integral valorization of BSG, sc-CO2 extraction and enzymatic hydrolysis processes have been applied to this by-product. First, the extraction of the lipophilic fraction of BSG with sc-CO2 has been systematically studied. The most influential extraction conditions, namely pressure and temperature, were varied from 20 to 40 MPa and from 313 to 353 K, respectively. A maximum yield of 5.70 ± 0.07 g /100 gBSG was obtained at 353 K and 40 MPa (see Graphical abstract, Fig. a)). High pressures and temperatures resulted in higher content of total phenolic and flavonoids compounds, as well as higher antioxidant capacity. Enzymatic hydrolysis was performed on samples after sc-CO2 treatment and non-treated BSG samples. Enzymatic hydrolysis was carried out at 323 K in an acetate buffer at pH=5 with a cellulase, 1,4-(1,3:1,4)-β-D-Glucan 4-glucanohydrolase, EC 3.2.1.4, from Aspergillus niger provided by Sigma-Aldrich. The graphical abstract b) represents the glucose yield along enzymatic hydrolysis for sc- CO2 treated and non-treated BSG at different enzyme concentrations. An improvement of the enzymatic hydrolysis yield by cellulase was observed in the sc-CO2 treated BSG compared to the non-treated. This improvement could be partially attributed to the removal of the lipid fraction and to morphological changes of BSG after sc-CO2. Based on this double benefit, sc-CO2 can play an important role on biomass valorization [8]. AEI [PID2019-104950RB-I00 and PID2020-116716RJ-I00] and JCyL and ERDF [BU050P20]. P. AlonsoRiaño and E. Trigueros acknowledge funding from JCyL and ESF [Orden EDU/556/2019 and EDU/574/2018, respectively]. R. Melgosa is supported by a Beatriz Galindo Research Fellowship [BG20/00182]. Ó. Benito-Román post-doctoral contract was funded by AEI [PID2020-116716RJ-I00/AEI/10.13039/501100011033]. A.E. Illera post-doctoral contract was funded by JCyL and ERDF [BU050P20]. application/pdf eng Ingeniería química Chemical engineering Supercritical carbon dioxide extraction of brewer´s spent grain (BSG) info:eu-repo/semantics/conferenceObject info:eu-repo/semantics/acceptedVersion info:eu-repo/semantics/openAccess THUMBNAIL Poster-Alonso_2022(1).pdf.jpg Poster-Alonso_2022(1).pdf.jpg IM Thumbnail image/jpeg 5721 https://riubu.ubu.es/bitstream/10259/6657/3/Poster-Alonso_2022%281%29.pdf.jpg c011b13e0aac6a4be65987eafe109b16 MD5 3 LICENSE license.txt license.txt text/plain; charset=utf-8 999 https://riubu.ubu.es/bitstream/10259/6657/2/license.txt b295bcbce42e2caabeb0c623d3860c06 MD5 2 ORIGINAL Poster-Alonso_2022(1).pdf Poster-Alonso_2022(1).pdf application/pdf 1839279 https://riubu.ubu.es/bitstream/10259/6657/1/Poster-Alonso_2022%281%29.pdf 5632ecc844d6cefc26db1648f95724cd MD5 1 10259/6657 oai:riubu.ubu.es:10259/6657 2022-05-20 15:22:04.173 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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