2026-04-20T11:35:37Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/66582022-05-20T13:21:59Zcom_10259_4244com_10259_5086com_10259_2604col_10259_4569

Alonso Riaño, Patricia aad9756c-ad87-4f31-ba02-459668ce3469 600 Melgosa Gómez, Rodrigo 357 600 Trigueros Andrés, Ester 557 600 Beltrán Calvo, Sagrario 57 600 Sanz Díez, Mª Teresa 530 600 2022-05-16T09:15:00Z 2022-05-16T09:15:00Z 2022 http://hdl.handle.net/10259/6658 Póster presentado en: EIFS2022, the 2nd Iberian Meeting on Supercritical Fluids (2º Encontro Ibérico de Fluidos Supercríticos / 2º Encuentro Ibérico de Fluidos Supercríticos), to be held on 28.February - 2.March 2022 in Coimbra, Portugal. Brewer ́s spent grain (BSG) is the solid by-product generated in breweries after the mashing and wort filtration process. It comprises about 85 % of the total by-products, generating approximately 20 kg per 100 L [1]. BSG presents a valuable chemical composition with a high content of protein and carbohydrates, 5 % of lipids and an important source of phenolic compounds. Among the different techniques proposed to valorize BSG, supercritical CO2 (sc-CO2) has become a promising technology to process biomass. The main aim of this work was to assess the effect of sc-CO2, after BSG oil extraction, on the subsequent enzymatic hydrolysis to hydrolyze the polysaccharide fraction into monomeric sugars by comparing the sugar yields of sc-CO2 and non-scCO2 treated BSG. After sc-CO2 treatment at 40 MPa and 80 oC, the raffinate obtained was subjected to enzymatic hydrolysis by cellulase at different enzyme dose. At the sc-CO2 extraction the carbohydrate fraction remained in the raffinate phase after extraction. Glucose yield increased with enzyme concentration for non-treated and sc-CO2 treated BSG. Higher yields of glucose were obtained for sc-CO2 treated compared to non-treated sc-CO2 for all the enzymes concentrations. The percentage increase in glucose yield for sc-CO2 treated and non-treated BSG was 8, 14 and 18 % for the three cellulase concentrations essayed in this work, 0.25, 0.5 and 1 %, respectively. The hydrolysis kinetics for xylose and arabinose have been also determined for non-treated and sc-CO2 treated BSG at 1 % of cellulase dose. Although not big differences were observed in the final sugar yield in the hydrolysates, the initial hydrolysis rate were significant higher for the sc-CO2 treated BSG than for the untreated samples. The higher enzymatic hydrolysis rate and yield obtained in the raffinate-BSG after sc- CO2 treatment compared with non-treated BSG could be attributed partially to the removal of the lipid fraction. As it has been described in literature [2], fats and oils could influence the susceptibility of carbohydrates to enzymes. This improvement was also due to surface morphology modification. The structural and chemical changes of untreated and sc-CO2 treated BSG was assessed by scanning electron microscopy and X-ray powder diffraction (XRD). Although the XRD pattern after sc-CO2 treatment indicated that the pre-treatment was not strong enough to modify the BSG crystallinity, the raffinate exhibited an irregular porosity and lamellar structure. sc-CO2 broke partially some structural barriers allowing a better enzyme access. Furthermore, some phenolic compounds were determined in the enzymatic hydrolysates at the end of the hydrolysis by using 1 % of cellulase (Table 1). It was observed that for p-coumaric acid and ferulic acid, a concentration 31 and 24 % higher, respectively, was obtained in the BSG hydrolysates after sc-CO2 treatment, while for vanillin similar concentration was obtained in both hydrolysates. The concentration of p-coumaric acid and vanillin after cellulase hydrolysis was lower than the values previously reported for the same BSG by alkaline, xylanase (1%) and subcritical water hydrolysis [3], [4]. Nevertheless, for ferulic acid, only a higher value was reached for alkaline hydrolysis. JCyL and ERDF for financial support of project BU050P20 Agencia Estatal de Investigación for financial support of project PID2019-104950RB-I00 / AEI / 10.13039/501100011033 JCyL and ESF for E. Trigueros (ORDEN EDU/574/2018) and P. Alonso-Riaño predoctoral (EDU/556/2019) contracts R. Melgosa is supported by a Beatriz Galindo Research Fellowship [BG20/00182] application/pdf eng Ingeniería química Chemical engineering Modification of brewer’s spent grain after sc-CO2 extraction: improvement of sugar and phenolic compounds release info:eu-repo/semantics/conferenceObject info:eu-repo/semantics/acceptedVersion info:eu-repo/semantics/openAccess THUMBNAIL Poster-Alonso_2022(2).pdf.jpg Poster-Alonso_2022(2).pdf.jpg IM Thumbnail image/jpeg 6536 https://riubu.ubu.es/bitstream/10259/6658/3/Poster-Alonso_2022%282%29.pdf.jpg 0393f6bb9cd87c4fe825b3aecf8a5689 MD5 3 LICENSE license.txt license.txt text/plain; charset=utf-8 999 https://riubu.ubu.es/bitstream/10259/6658/2/license.txt b295bcbce42e2caabeb0c623d3860c06 MD5 2 ORIGINAL Poster-Alonso_2022(2).pdf Poster-Alonso_2022(2).pdf application/pdf 796454 https://riubu.ubu.es/bitstream/10259/6658/1/Poster-Alonso_2022%282%29.pdf 893a67eb1ab702debabaa8f248cf77f2 MD5 1 10259/6658 oai:riubu.ubu.es:10259/6658 2022-05-20 15:21:59.635 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es RWwgYXV0b3IgY29tbyDDum5pY28gdGl0dWxhciBkZSBsb3MgZGVyZWNob3MgZGUgcHJvcGllZGFkIGludGVsZWN0dWFsIGRlIGxhIG9icmEsIG8gZGlzcG9uaWVuZG8gZGUgbG9zIGRlYmlkb3MgcGVybWlzb3MgZGUgbG9zIG90cm9zIHRpdHVsYXJlcywgc2kgbG9zIGh1YmllcmEsIHkgZW4gdmlydHVkIGRlIGxvcyBkZXJlY2hvcyBxdWUgbGUgY29uZmllcmUgbGEgbGVnaXNsYWNpw7NuIHZpZ2VudGUgc29icmUgcHJvcGllZGFkIGludGVsZWN0dWFsIHkgZGVyZWNob3MgZGUgYXV0b3IsIA0KQVVUT1JJWkEgYSBsYSBVbml2ZXJzaWRhZCBkZSBCdXJnb3MgYSBkaWZ1bmRpciwgZGUgbWFuZXJhIGdyYXR1aXRhLCBlbCBjb250ZW5pZG8gZGUgbG9zIGFyY2hpdm9zIGRpZ2l0YWxlcyBxdWUgY29ycmVzcG9uZGVuIGFsIGRvY3VtZW50byBkZXNjcml0byBhbnRlcmlvcm1lbnRlLCBjb24gY2Fyw6FjdGVyIG5vIGV4Y2x1c2l2byB5IGRlIG1hbmVyYSBww7pibGljYSBlbiBhY2Nlc28gYWJpZXJ0byBhIHRyYXbDqXMgZGUgSW50ZXJuZXQsIHBhcmEgbG8gcXVlIGxhIEJpYmxpb3RlY2EgcHJvY2VkZXLDoSBhIGFyY2hpdmFybG9zIGVuIGVsIFJlcG9zaXRvcmlvIEluc3RpdHVjaW9uYWwuIEFzaW1pc21vIGF1dG9yaXphIGEgbGEgVW5pdmVyc2lkYWQgZGUgQnVyZ29zIGEgcmVhbGl6YXIgbGFzIHRyYW5zZm9ybWFjaW9uZXMgbmVjZXNhcmlhcyBkZSBmb3JtYXRvLCBubyBkZSBjb250ZW5pZG8sIHBhcmEgZ2FyYW50aXphciBsYSBwcmVzZXJ2YWNpw7NuIHkgZWwgYWNjZXNvIGVuIGVsIGZ1dHVyby4NCg0KRWwgYXV0b3IgZGlzcG9uZSwgZW4gdG9kbyBjYXNvLCBkZWwgZGVyZWNobyBhIHJldm9jYXIgZXN0YSBhdXRvcml6YWNpw7NuLg0KDQpMYSBjZXNpw7NuIGRlIGRlcmVjaG9zIGRlIGVzdGEgb2JyYSBzZSBlbmN1ZW50cmEgc3VqZXRhIGEgbGEgbGVnaXNsYWNpw7NuIHZpZ2VudGUgc29icmUgcHJvcGllZGFkIGludGVsZWN0dWFsIHkgZGVyZWNob3MgZGUgYXV0b3Iu