2026-04-29T08:25:32Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/66852022-06-24T11:00:39Zcom_10259_4313com_10259_5086com_10259_2604col_10259_4314

Arnáiz Alonso, Ana 9e0db041-94eb-4581-8747-f72f3a416691 Guembe García, Marta 9c4f2b3d-87cf-461b-b10e-563a95dabb53 600 Delgado-Pinar, Estefanía fdc2fe9d-0c78-4795-b719-54954121831c Valente, Artur J. M. f63e6b05-eaee-4126-9d18-bca6f10d3b31 600 Ibeas Cortes, Saturnino 293 600 García Pérez, José Miguel 213 600 Vallejos Calzada, Saúl 567 600 2022-05-30T12:37:10Z 2022-05-30T12:37:10Z 2022-05 http://hdl.handle.net/10259/6685 10.1038/s41598-022-12848-4 2045-2322 We have faced the preparation of fully water-soluble fluorescent peptide substrate with long-term environmental stability (in solution more than 35 weeks) and, accordingly, with stable results in the use of this probe in determining the activity of enzymes. We have achieved this goal by preparing a co-polymer of the commercial N-vinyl-2-pyrrolidone (99.5% mol) and a fluorescent substrate for trypsin activity determination having a vinylic group (0.5%). The activity of trypsin has been measured in water solutions of this polymer over time, contrasted against the activity of both the commercial substrate Z-L-Arg-7-amido-4-methylcoumarin hydrochloride and its monomeric derivative, prepared ad-hoc. Initially, the activity of the sensory polymer was 74.53 ± 1.72 nmol/min/mg of enzyme, while that of the commercial substrate was 20.44 ± 0.65 nmol/min/mg of enzyme, the former maintained stable along weeks and the latter with a deep decay to zero in three weeks. The ‘protection’ effect exerted by the polymer chain has been studied by solvation studies by UV–Vis spectroscopy, steady-state & time resolved fluorescence, thermogravimetry and isothermal titration calorimetry. "La Caixa" Foundation, under agreement LCF/PR/PR18/51130007. We also gratefully acknowledge the financial support provided by FEDER (Fondo Europeo de Desarrollo Regional) through Programa Operacional Factores de Competitividade (COMPETE), and the Spanish State Research Agency (PID2020-113264RB-I00/AEI/10.13039/501100011033). Centro de Química de Coimbra acknowledges Fundação para a Ciência e Tecnologia for financial support (Projects UIDB/00313/2020, UIDP/00313/2020). E.D.-P. thanks the “Concurso de Estímulo ao Emprego Científico” for the junior contract CEECIND/04136/2018. application/pdf eng Springer Nature Scientific Reports. 2022, V. 12, 8818 https://doi.org/10.1038/s41598-022-12848-4 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113264RB-I00/ES/DISEÑO, SINTESIS Y CARACTERIZACION DE POLIMEROS INTELIGENTES. APLICACION DE SENSORES QUIMICOS POLIMERICOS EN CONTROL Y SEGURIDAD ALIMENTARIA Y EN EL AMBITO SANITARIO info:eu-repo/grantAgreement/FCT//UIDB%2F00313%2F2020 info:eu-repo/grantAgreement/FCT//UIDP%2F00313%2F2020 Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Actuators Sensors and biosensors Química orgánica Chemistry, Organic The role of polymeric chains as a protective environment for improving the stability and efficiency of fluorogenic peptide substrates info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Scientific Reports 12 1 THUMBNAIL Arnaiz-sr_2022.pdf.jpg Arnaiz-sr_2022.pdf.jpg IM Thumbnail image/jpeg 3453 https://riubu.ubu.es/bitstream/10259/6685/4/Arnaiz-sr_2022.pdf.jpg d5b4f36452ebc64dc753b8be6848c413 MD5 4 LICENSE license.txt license.txt text/plain; charset=utf-8 998 https://riubu.ubu.es/bitstream/10259/6685/3/license.txt aa90105e1c90a1b4fe810649aa967b7d MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 908 https://riubu.ubu.es/bitstream/10259/6685/2/license_rdf 0175ea4a2d4caec4bbcc37e300941108 MD5 2 ORIGINAL Arnaiz-sr_2022.pdf Arnaiz-sr_2022.pdf application/pdf 3025343 https://riubu.ubu.es/bitstream/10259/6685/1/Arnaiz-sr_2022.pdf f418657726fa4ed4977d41509a4874c8 MD5 1 10259/6685 oai:riubu.ubu.es:10259/6685 2022-06-24 13:00:39.084 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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