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oai:riubu.ubu.es:10259/71412022-11-25T01:05:35Zcom_10259_5363com_10259_5086com_10259_2604com_10259_4313col_10259_5364col_10259_4314

2022-11-24T13:49:27Z urn:hdl:10259/7141 Straightforward purification method for the determination of the activity of glucose oxidase and catalase in honey by extracting polyphenols with a film-shaped polymer González Ceballos, Lara Guirado Moreno, José Carlos Utzeri, Gianluca García Pérez, José Miguel Fernández Muiño, Miguel Ángel Osés Gómez, Sandra María Sancho Ortiz, María Teresa Arnáiz Alonso, Ana Valente, Artur J. M. Vallejos Calzada, Saúl Catechol 1,2-Dihydroxybenzene Phenylboronic acid Glucose oxidase Catalase Honey Glucose oxidase (GOX) and catalase (CAT) regulate the amount of H2O2 in honey, by generating or consuming it, so they are related to the antibacterial and antioxidant activity of honey. However, their activities are hardly analysed, since the process requires a previous dialysis that is non-selective, very time-consuming (>24 h), eco-unfriendly (>6L of buffer) and expensive. This research shows the design and performance of a material that selectively removes the actual interferents. The film-shaped-polymer is immersed for 90́ within a honey solution (12.5 mL of buffer), where it interacts exclusively with 1,2-dihydroxybenzenes, which we proved to be the real interferents (the material contains motifs derived from phenylboronic acid to interact with 1,2-diols). Polymeric chains favour condensation to occur exclusively with 1,2-dihydroxybenzenes, excluding monosaccharides. The interferents’ removal using our designed polymer is selective, low cost (1.42€ per test), rapid and eco-friendly (saves 6L of buffer and 20.5 h of experimental workout per sample). 2022-11-24T13:49:27Z 2022-11-24T13:49:27Z 2023-03 info:eu-repo/semantics/article 0308-8146 http://hdl.handle.net/10259/7141 10.1016/j.foodchem.2022.134789 eng Food Chemistry. 2023, V. 405, Part A, 134789 https://doi.org/10.1016/j.foodchem.2022.134789 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113264RB-I00/ES/DISEÑO, SINTESIS Y CARACTERIZACION DE POLIMEROS INTELIGENTES. APLICACION DE SENSORES QUIMICOS POLIMERICOS EN CONTROL Y SEGURIDAD ALIMENTARIA Y EN EL AMBITO SANITARIO info:eu-repo/grantAgreement/FCT//SFR%2FBD%2F146358%2F2019 info:eu-repo/grantAgreement/FCT//UIDB%2F00313%2F2020 info:eu-repo/grantAgreement/FCT//UIDP%2F00313%2F2020 http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Atribución 4.0 Internacional Elsevier