2026-04-18T14:57:47Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/71412022-11-25T01:05:35Zcom_10259_5363com_10259_5086com_10259_2604com_10259_4313col_10259_5364col_10259_4314

00925njm 22002777a 4500 dc González Ceballos, Lara author Guirado Moreno, José Carlos author Utzeri, Gianluca author García Pérez, José Miguel author Fernández Muiño, Miguel Ángel author Osés Gómez, Sandra María author Sancho Ortiz, María Teresa author Arnáiz Alonso, Ana author Valente, Artur J. M. author Vallejos Calzada, Saúl author 2023-03 Glucose oxidase (GOX) and catalase (CAT) regulate the amount of H2O2 in honey, by generating or consuming it, so they are related to the antibacterial and antioxidant activity of honey. However, their activities are hardly analysed, since the process requires a previous dialysis that is non-selective, very time-consuming (>24 h), eco-unfriendly (>6L of buffer) and expensive. This research shows the design and performance of a material that selectively removes the actual interferents. The film-shaped-polymer is immersed for 90́ within a honey solution (12.5 mL of buffer), where it interacts exclusively with 1,2-dihydroxybenzenes, which we proved to be the real interferents (the material contains motifs derived from phenylboronic acid to interact with 1,2-diols). Polymeric chains favour condensation to occur exclusively with 1,2-dihydroxybenzenes, excluding monosaccharides. The interferents’ removal using our designed polymer is selective, low cost (1.42€ per test), rapid and eco-friendly (saves 6L of buffer and 20.5 h of experimental workout per sample). 0308-8146 http://hdl.handle.net/10259/7141 10.1016/j.foodchem.2022.134789 Catechol 1,2-Dihydroxybenzene Phenylboronic acid Glucose oxidase Catalase Honey Straightforward purification method for the determination of the activity of glucose oxidase and catalase in honey by extracting polyphenols with a film-shaped polymer