2026-04-18T14:56:24Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/71412022-11-25T01:05:35Zcom_10259_5363com_10259_5086com_10259_2604com_10259_4313col_10259_5364col_10259_4314
Repositorio Institucional de la Universidad de Burgos
author
González Ceballos, Lara
author
Guirado Moreno, José Carlos
author
Utzeri, Gianluca
author
García Pérez, José Miguel
author
Fernández Muiño, Miguel Ángel
author
Osés Gómez, Sandra María
author
Sancho Ortiz, María Teresa
author
Arnáiz Alonso, Ana
author
Valente, Artur J. M.
author
Vallejos Calzada, Saúl
2022-11-24T13:49:27Z
2022-11-24T13:49:27Z
2023-03
0308-8146
http://hdl.handle.net/10259/7141
10.1016/j.foodchem.2022.134789
Glucose oxidase (GOX) and catalase (CAT) regulate the amount of H2O2 in honey, by generating or consuming it, so they are related to the antibacterial and antioxidant activity of honey. However, their activities are hardly analysed, since the process requires a previous dialysis that is non-selective, very time-consuming (>24 h), eco-unfriendly (>6L of buffer) and expensive. This research shows the design and performance of a material that selectively removes the actual interferents. The film-shaped-polymer is immersed for 90́ within a honey solution (12.5 mL of buffer), where it interacts exclusively with 1,2-dihydroxybenzenes, which we proved to be the real interferents (the material contains motifs derived from phenylboronic acid to interact with 1,2-diols). Polymeric chains favour condensation to occur exclusively with 1,2-dihydroxybenzenes, excluding monosaccharides. The interferents’ removal using our designed polymer is selective, low cost (1.42€ per test), rapid and eco-friendly (saves 6L of buffer and 20.5 h of experimental workout per sample).
eng
Atribución 4.0 Internacional
Catechol
1,2-Dihydroxybenzene
Phenylboronic acid
Glucose oxidase
Catalase
Honey
Straightforward purification method for the determination of the activity of glucose oxidase and catalase in honey by extracting polyphenols with a film-shaped polymer
info:eu-repo/semantics/article
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URL
https://riubu.ubu.es/bitstream/10259/7141/1/Gonzalez-fc_2023.pdf
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2db82aef57e2955a811af8bf55e175ec
2058347
application/pdf
Gonzalez-fc_2023.pdf