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oai:riubu.ubu.es:10259/73162023-01-26T01:05:25Zcom_10259_3592com_10259_3591com_10259.4_106com_10259_2604com_10259_6168com_10259_5086com_10259_4313col_10259_3930col_10259_6169col_10259_4314

2023-01-25T12:38:53Z urn:hdl:10259/7316 Lab-on-a-chip for the easy and visual detection of SARS-CoV-2 in saliva based on sensory polymers Arnáiz Alonso, Ana Guirado Moreno, José Carlos Guembe García, Marta Barros García, Rocío Tamayo Ramos, Juan Antonio Fernández Pampín, Natalia García Pérez, José Miguel Vallejos Calzada, Saúl Peptide Substrate Fluorimetry Immobilization COVID-19 Paper-supported Coating Pandemics The initial stages of the pandemic caused by SARS-CoV-2 showed that early detection of the virus in a simple way is the best tool until the development of vaccines. Many different tests are invasive or need the patient to cough up or even drag a sample of mucus from the throat area. Besides, the manufacturing time has proven insufficient in pandemic conditions since they were out of stock in many countries. Here we show a new method of manufacturing virus sensors and a proof of concept with SARS-CoV-2. We found that a fluorogenic peptide substrate of the main protease of the virus (Mpro) can be covalently immobilized in a polymer, with which a cellulose-based material can be coated. These sensory labels fluoresce with a single saliva sample of a positive COVID-19 patient. The results matched with that of the antigen tests in 22 of 26 studied cases (85% success rate). 2023-01-25T12:38:53Z 2023-01-25T12:38:53Z 2023-03 info:eu-repo/semantics/article 0925-4005 http://hdl.handle.net/10259/7316 10.1016/j.snb.2022.133165 eng Sensors and Actuators B: Chemical. 2023, V. 379, 133165 https://doi.org/10.1016/j.snb.2022.133165 info:eu-repo/grantAgreement/Fundación Bancaria Caixa d'Estalvis i Pensions de Barcelona//LCF%2FPR%2FPR18%2F51130007/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113264RB-I00/ES/DISEÑO, SINTESIS Y CARACTERIZACION DE POLIMEROS INTELIGENTES. APLICACION DE SENSORES QUIMICOS POLIMERICOS EN CONTROL Y SEGURIDAD ALIMENTARIA Y EN EL AMBITO SANITARIO/ http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Atribución 4.0 Internacional Elsevier