2026-04-17T11:43:52Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/73162023-01-26T01:05:25Zcom_10259_3592com_10259_3591com_10259.4_106com_10259_2604com_10259_6168com_10259_5086com_10259_4313col_10259_3930col_10259_6169col_10259_4314

00925njm 22002777a 4500 dc Arnáiz Alonso, Ana author Guirado Moreno, José Carlos author Guembe García, Marta author Barros García, Rocío author Tamayo Ramos, Juan Antonio author Fernández Pampín, Natalia author García Pérez, José Miguel author Vallejos Calzada, Saúl author 2023-03 The initial stages of the pandemic caused by SARS-CoV-2 showed that early detection of the virus in a simple way is the best tool until the development of vaccines. Many different tests are invasive or need the patient to cough up or even drag a sample of mucus from the throat area. Besides, the manufacturing time has proven insufficient in pandemic conditions since they were out of stock in many countries. Here we show a new method of manufacturing virus sensors and a proof of concept with SARS-CoV-2. We found that a fluorogenic peptide substrate of the main protease of the virus (Mpro) can be covalently immobilized in a polymer, with which a cellulose-based material can be coated. These sensory labels fluoresce with a single saliva sample of a positive COVID-19 patient. The results matched with that of the antigen tests in 22 of 26 studied cases (85% success rate). 0925-4005 http://hdl.handle.net/10259/7316 10.1016/j.snb.2022.133165 Peptide Substrate Fluorimetry Immobilization COVID-19 Paper-supported Coating Pandemics Lab-on-a-chip for the easy and visual detection of SARS-CoV-2 in saliva based on sensory polymers