2026-04-17T10:24:49Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/73162023-01-26T01:05:25Zcom_10259_3592com_10259_3591com_10259.4_106com_10259_2604com_10259_6168com_10259_5086com_10259_4313col_10259_3930col_10259_6169col_10259_4314

Arnáiz Alonso, Ana Guirado Moreno, José Carlos Guembe García, Marta Barros García, Rocío Tamayo Ramos, Juan Antonio Fernández Pampín, Natalia García Pérez, José Miguel Vallejos Calzada, Saúl 2023-01-25T12:38:53Z 2023-01-25T12:38:53Z 2023-03 0925-4005 http://hdl.handle.net/10259/7316 10.1016/j.snb.2022.133165 The initial stages of the pandemic caused by SARS-CoV-2 showed that early detection of the virus in a simple way is the best tool until the development of vaccines. Many different tests are invasive or need the patient to cough up or even drag a sample of mucus from the throat area. Besides, the manufacturing time has proven insufficient in pandemic conditions since they were out of stock in many countries. Here we show a new method of manufacturing virus sensors and a proof of concept with SARS-CoV-2. We found that a fluorogenic peptide substrate of the main protease of the virus (Mpro) can be covalently immobilized in a polymer, with which a cellulose-based material can be coated. These sensory labels fluoresce with a single saliva sample of a positive COVID-19 patient. The results matched with that of the antigen tests in 22 of 26 studied cases (85% success rate). eng http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Atribución 4.0 Internacional Peptide Substrate Fluorimetry Immobilization COVID-19 Paper-supported Coating Pandemics Lab-on-a-chip for the easy and visual detection of SARS-CoV-2 in saliva based on sensory polymers info:eu-repo/semantics/article