2026-04-17T10:28:50Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/73162023-01-26T01:05:25Zcom_10259_3592com_10259_3591com_10259.4_106com_10259_2604com_10259_6168com_10259_5086com_10259_4313col_10259_3930col_10259_6169col_10259_4314
Arnáiz Alonso, Ana
762
600
0000-0002-3842-498X
Guirado Moreno, José Carlos
770
600
0000-0001-6233-5496
Guembe García, Marta
769
600
0000-0003-2483-2576
Barros García, Rocío
787
600
0000-0002-0392-8504
Tamayo Ramos, Juan Antonio
547
600
0000-0002-7071-002X
Fernández Pampín, Natalia
b774f214-fa4c-441c-926c-8b54518b68cd
600
García Pérez, José Miguel
213
600
0000-0002-2674-8194
Vallejos Calzada, Saúl
567
600
0000-0001-5522-6574
2023-01-25T12:38:53Z
2023-01-25T12:38:53Z
2023-03
0925-4005
http://hdl.handle.net/10259/7316
10.1016/j.snb.2022.133165
The initial stages of the pandemic caused by SARS-CoV-2 showed that early detection of the virus in a simple way is the best tool until the development of vaccines. Many different tests are invasive or need the patient to cough up or even drag a sample of mucus from the throat area. Besides, the manufacturing time has proven insufficient in pandemic conditions since they were out of stock in many countries. Here we show a new method of manufacturing virus sensors and a proof of concept with SARS-CoV-2. We found that a fluorogenic peptide substrate of the main protease of the virus (Mpro) can be covalently immobilized in a polymer, with which a cellulose-based material can be coated. These sensory labels fluoresce with a single saliva sample of a positive COVID-19 patient. The results matched with that of the antigen tests in 22 of 26 studied cases (85% success rate).
We gratefully acknowledge the financial support provided by all funders. Author Saul Vallejos coordinates the project leading to these results, which has received funding from "La Caixa" Foundation, under agreement LCF/PR/PR18/51130007. This work was supported by the Regional Government of Castilla y León (Junta de Castilla y León) and by the Ministry of Science and Innovation MICIN and the European Union NextGenerationEU PRTR. Author Jose Miguel García received grant PID2020–113264RB-I00 funded by MCIN/AEI/ 10.13039/501100011033 and by “ERDF A way of making Europe”. Ana Arnaiz received funding from Ministerio de Universidades-European Union in the frame of NextGenerationEU RD 289/2021 (Universidad Politécnica de Madrid). Finally, all the authors want to thank the support provided by City Hall of Villadiego “Ayuntamiento de Villadiego” when looking for participants for the proof of concept.
application/pdf
eng
Elsevier
Sensors and Actuators B: Chemical. 2023, V. 379, 133165
https://doi.org/10.1016/j.snb.2022.133165
info:eu-repo/grantAgreement/Fundación Bancaria Caixa d'Estalvis i Pensions de Barcelona//LCF%2FPR%2FPR18%2F51130007/
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113264RB-I00/ES/DISEÑO, SINTESIS Y CARACTERIZACION DE POLIMEROS INTELIGENTES. APLICACION DE SENSORES QUIMICOS POLIMERICOS EN CONTROL Y SEGURIDAD ALIMENTARIA Y EN EL AMBITO SANITARIO/
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Peptide
Substrate
Fluorimetry
Immobilization
COVID-19
Paper-supported
Coating
Pandemics
Química orgánica
Enfermedades infecciosas
Chemistry, Organic
Communicable diseases
Lab-on-a-chip for the easy and visual detection of SARS-CoV-2 in saliva based on sensory polymers
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Sensors and Actuators B: Chemical
379
133165
THUMBNAIL
Arnaiz-sabc_2023.pdf.jpg
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MD5
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LICENSE
license.txt
license.txt
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https://riubu.ubu.es/bitstream/10259/7316/3/license.txt
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ORIGINAL
Arnaiz-sabc_2023.pdf
Arnaiz-sabc_2023.pdf
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https://riubu.ubu.es/bitstream/10259/7316/1/Arnaiz-sabc_2023.pdf
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10259/7316
oai:riubu.ubu.es:10259/7316
2023-01-26 02:05:25.837
Repositorio Institucional de la Universidad de Burgos
bubrep@ubu.es
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