2026-04-20T10:05:27Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/74232023-03-28T10:31:18Zcom_10259_6168com_10259_5086com_10259_2604col_10259_6169

Evaluation of biostimulation, bioaugmentation, and organic amendments application on the bioremediation of recalcitrant hydrocarbons of soil Curiel Alegre, Sandra Velasco Arroyo, Blanca Rumbo Lorenzo, Carlos Khan, Aqib Hassan Ali Tamayo Ramos, Juan Antonio Rad Moradillo, Juan Carlos Gallego, José Luis Barros García, Rocío Biostimulation Bioaugmentation Phospholipid fatty acids Total petroleum hydrocarbons polluted soils Soil enzymes Materiales Materials In the present work, the operational conditions for improving the degradation rates of Total Petroleum Hydrocarbons (TPHs) in contaminated soil from a machinery park were optimized at a microcosms scale along a 90- days incubation period. In this study, bioremediation strategies and an organic amendment have been tested to verify the remediation of soil contaminated with different hydrocarbons, mineral oils, and heavy metals. Specifically, designed biostimulation and bioaugmentation strategies were compared with and without adding vermicompost. The polluted soil harboring multiple contaminants, partially attenuated for years, was used. The initial profile showed enrichment in heavy linear alkanes, suggesting a previous moderate weathering. The application of vermicompost increased five and two times the amounts of available phosphorus (P) and exchangeable potassium (K), respectively, as a direct consequence of the organic amendment addition. The microbial activity increased due to soil acidification, which influenced the solubility of P and other micronutrients. It also impacted the predominance and variability of the different microbial groups and the incubation, as reflected by phospholipid fatty acid (PLFA) results. An increase in the alkaline phosphatases and proteases linked to bacterial growth was displayed. This stimulation of microbial metabolism correlated with the degradation rates since TPHs degradation’ efficiency after vermicompost addition reached 32.5% and 34.4% of the initial hydrocarbon levels for biostimulation and bioaugmentation, respectively. Although Polycyclic Aromatic Hydrocarbons (PAHs) were less abundant in this soil, results also decreased, especially for the most abundant, the phenanthrene. Despite improving the degradation rates, results revealed that recalcitrant and hydrophobic petroleum compounds remained unchanged, indicating that mobility, linked to bioavailability, probably represents the limiting step for further soil recovery. This work is funded by the GREENER project of the European Union’s Horizon 2020 research and innovation program (Grant Agreement No. 826312). S. Curiel pre-doctoral contract was funded by Junta de Castilla y Leon ´ (ORDEN EDU/1508/2020, de 15 de diciembre). 2023-02-08T11:50:30Z 2023-02-08T11:50:30Z 2022-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 0045-6535 http://hdl.handle.net/10259/7423 10.1016/j.chemosphere.2022.135638 eng Chemosphere. 2022, V. 307, 135638 https://doi.org/10.1016/j.chemosphere.2022.135638 info:eu-repo/grantAgreement/EC/H2020/826312/EU/InteGRated systems for Effective ENvironmEntal Remediation/GREENER/ Atribución-NoComercial 4.0 Internacional http://creativecommons.org/licenses/by-nc/4.0/ info:eu-repo/semantics/openAccess application/pdf Elsevier https://riubu.ubu.es/bitstream/10259/7423/4/Curiel-c_2022.pdf.jpg Hispana TEXT http://creativecommons.org/licenses/by-nc/4.0/ RIUBU. Repositorio Institucional de la Universidad de Burgos http://hdl.handle.net/10259/7423