2026-05-23T20:13:44Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/75072023-04-18T09:56:28Zcom_10259_4862com_10259_5086com_10259_2604col_10259_4863
Graff Reis, Jacqueline
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Dai Prá, Isabella
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Michelon, William
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Viancelli, Aline
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Piedrahita Marquez, David Guillermo
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Schmitz, Caroline
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Maraschin, Marcelo
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Moura, Sidnei
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Thaís Silva, Izabella
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Oliveira Costa, Geovanna de
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Tizziani, Tiago
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Sandjo, Louis P.
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Rodríguez Lázaro, David
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0000-0002-8795-854X
Fongaro, Gislaine
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2023-03-08T08:13:19Z
2023-03-08T08:13:19Z
2022-11
http://hdl.handle.net/10259/7507
10.3390/ijerph192315823
1660-4601
Certain members of the Coronaviridae family have emerged as zoonotic agents and
have recently caused severe respiratory diseases in humans and animals, such as SARS, MERS,
and, more recently, COVID-19. Antivirals (drugs and antiseptics) capable of controlling viruses at
the site of infection are scarce. Microalgae from the Chlorellaceae family are sources of bioactive
compounds with antioxidant, antiviral, and antitumor activity. In the present study, we aimed to
evaluate various extracts from Planktochlorella nurekis in vitro against murine coronavirus-3 (MHV-3),
which is an essential human coronavirus surrogate for laboratory assays. Methanol, hexane, and
dichloromethane extracts of P. nurekis were tested in cells infected with MHV-3, and characterized
by UV-vis spectrophotometry, nuclear magnetic resonance (NMR) spectroscopy, ultraperformance
liquid chromatography-mass spectrometry (UPLC-MS), and the application of chemometrics through
principal component analysis (PCA). All the extracts were highly efficient against MHV-3 (more than
a 6 Log unit reduction), regardless of the solvent used or the concentration of the extract, but the
dichloromethane extract was the most effective. Chemical characterization by spectrophotometry
and NMR, with the aid of statistical analysis, showed that polyphenols, carbohydrates, and isoprene
derivatives, such as terpenes and carotenoids have a more significant impact on the virucidal potential.
Compounds identified by UPLC-MS were mainly lipids and only found in the dichloromethane
extract. These results open new biotechnological possibilities to explore the biomass of P. nurekis; it is
a natural extract and shows low cytotoxicity and an excellent antiviral effect, with low production
costs, highlighting a promising potential for development and implementation of therapies against
coronaviruses, such as SARS-CoV-2.
This research was funded LVA-MIP-CCB-UFSC/Sigpex: 201917940, and CNPq, CAPES-DS.
application/pdf
eng
MDPI
International Journal of Environmental Research and Public Health. 2022, V. 19, n. 23, 15823
https://doi.org/10.3390/ijerph192315823
info:eu-repo/grantAgreement/UFSC//201917940/BR/
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Coronavirus
SARS-CoV-2
Murine coronavirus
Metabolomic approach
NMR biomarkers
UPLC-MS
Viricidal activity
Antiviral activity
Microbiología
Microbiology
Characterization of Planktochlorella nurekis Extracts and Virucidal Activity against a Coronavirus Model, the Murine Coronavirus 3
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
International Journal of Environmental Research and Public Health
19
23
THUMBNAIL
Graff-ijerph_2022.pdf.jpg
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LICENSE
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license.txt
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https://riubu.ubu.es/bitstream/10259/7507/3/license.txt
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ORIGINAL
Graff-ijerph_2022.pdf
Graff-ijerph_2022.pdf
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https://riubu.ubu.es/bitstream/10259/7507/1/Graff-ijerph_2022.pdf
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10259/7507
oai:riubu.ubu.es:10259/7507
2023-04-18 11:56:28.426
Repositorio Institucional de la Universidad de Burgos
bubrep@ubu.es
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