2026-04-21T06:46:44Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/75262023-04-18T10:38:27Zcom_10259_4244com_10259_5086com_10259_2604com_10259_7524com_10259.4_2512com_10259.4_106col_10259_4245col_10259_7525
Subcritical Water as Pretreatment Technique for Bioethanol Production from Brewer’s Spent Grain within a Biorefinery Concept
Alonso Riaño, Patricia
Amândio, Mariana S.T.
Xavier, Ana María Rebelo Barreto
Beltrán Calvo, Sagrario
Sanz Díez, Mª Teresa
Subcritical water pretreatment
Brewer's spent grain
Lignocellulosic biomass
High solids' loading
Simultaneous saccharification and fermentation
Separate hydrolysis and fermentation
Bioeconomy and environmental issues envisage industrial by-products such as Brewer’s
spent grain (BSG) as renewable resources for their recycling and reuse within a biorefinery concept.
This study aimed to investigate the production of bioethanol from subcritical water (subW) pretreated
BSG, following the conversion of the BSG biopolymers cellulose and hemicelluloses. The subW
pretreatment was performed in a batch reactor at 174 ◦C, during 60 min and 5% (w/v) of dry BSG
charge. The behavior of BSG biopolymers under subW pretreatment was monitored by evaluating
the chemical composition of the liquid and solid streams and the chemical and structural changes
caused in the solid residues by scanning electron microscope (SEM), CHNS elemental analysis
and water retention value (WRV). The production of bioethanol from subW-pretreated BSG was
assessed by separate hydrolysis and fermentation (SHF) and also by simultaneous saccharification
and fermentation (SSF) by using the enzymatic cocktail Celluclast 1.5 L (40 FPU/gsolids) and the yeast
Ethanol Red®. The higher bioethanol productivity (1.073 g·L
−1
·h
−1
) and concentration (32.18 g/L)
were achieved by SSF with higher solids’ loading (25%) and following a fed-batch strategy. These
results suggest that subcritical water pretreatment is a promising technology for the valorization of
BSG as a feedstock for second-generation bioethanol production.
2023-03-10T10:59:50Z
2023-03-10T10:59:50Z
2023-03-10T10:59:50Z
2022-11
info:eu-repo/semantics/article
http://hdl.handle.net/10259/7526
10.3390/polym14235218
2073-4360
eng
Polymers. 2022, V. 14, n. 23, 5218
https://doi.org/10.3390/polym14235218
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104950RB-I00/ES/VALORIZACION DE SUBPRODUCTOS MARINOS MEDIANTE TECNOLOGIAS DE FLUIDOS SUB- Y SUPERCRITICOS PARA LA OBTENCION DE BIOCOMPUESTOS VALIOSOS/
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica, Técnica y de Innovación 2021-2023/TED2021-129311B-I00/ES/Tecnologías verdes basadas en el empleo de fluidos presurizados para valorizar biomasa lignocelulósica mediante la producción de furfural/
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica, Técnica y de Innovación 2021-2023/PDC2022-133443-I00/ES/Diseño de un prototipo para la extracción de bioproductos con agua subcrítica/
info:eu-repo/grantAgreement/Junta de Castilla y León//BU050P20//Valorización de la fracción proteica de subproductos de la industria agroalimentaria de Castilla y León mediante tecnologías de fluidos presurizados/
info:eu-repo/grantAgreement/FCT//UIDP%2F50011%2F2020/PT/
info:eu-repo/grantAgreement/FCT//LA%2FP%2F0006%2F2020/PT/
info:eu-repo/grantAgreement/FCT//UIDB%2F50011%2F2020/PT/
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Atribución 4.0 Internacional
MDPI