2026-04-19T06:28:35Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/75942023-03-25T01:05:22Zcom_10259.4_2513com_10259.4_2512com_10259.4_106com_10259_2604com_10259.4_2516com_10259_5086col_10259.4_2514col_10259.4_2517

Busto Núñez, Mª Dolores González Temiño, Yaiza Albillos García, Silvia María Ramos Gómez, Sonia Pilar Izquierdo, María Concepción Palacios Santamaría, David Ortega Santamaría, Natividad 2023-03-24T08:41:52Z 2023-03-24T08:41:52Z 2022-07 http://hdl.handle.net/10259/7594 10.3390/foods11142077 2304-8158 In this study, the use of spray-drying technology for encapsulating Flavourzyme® (protease– peptidase complex) was evaluated to overcome the limitations (low encapsulation efficiency and no large-scale production) of other encapsulation processes. To the best of our knowledge, spray drying has not been applied previously for the immobilization of this enzyme. Firstly, bovine serum albumin (BSA), as a model protein, was encapsulated by spray drying in chitosan and tripolyphoshate (TPP) cross-linked-chitosan shell matrices. The results showed that the chitosan–TPP microcapsules provided a high encapsulation efficiency and better protein stability compared to the non-crosslinked chitosan microcapsules. The effect of enzyme concentration and drying temperature were tested during the spray drying of Flavourzyme®. In this regard, an activity yield of 88.0% and encapsulation efficiency of 78.6% were obtained with a concentration of 0.1% (v/v) and an inlet temperature of 130 ◦C. Flavourzyme®-loaded chitosan microcapsules were also characterized in terms of their size and morphology using scanning electron microscopy and laser diffractometry application/pdf eng MDPI Foods. 2022, V. 11, n. 14, 2077 https://doi.org/10.3390/foods11142077 Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Chitosan Flavourzyme® Microencapsulation Protease Spray drying Tripolyphosphate Bioquímica Alimentos Biochemistry Food Microencapsulation of a Commercial Food-Grade Protease by Spray Drying in Cross-Linked Chitosan Particles info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Foods 11 14