2026-06-30T01:14:57Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/77142023-06-22T00:05:15Zcom_10259_4313com_10259_5086com_10259_2604col_10259_4314
2023-06-21T11:57:21Z
urn:hdl:10259/7714
Isolation and Quantification of Mandelonitrile from Arabidopsis thaliana Using Gas Chromatography/Mass Spectrometry
Arnáiz Alonso, Ana
Vallejo García, Jorge Lucas
Vallejos Calzada, Saúl
Diaz, Isabel
Benzaldehyde cyanohydrin
Mandelonitrile
Hydroxynitrile lyase
Arabidopsis thaliana
Spider mite
Cyanogenic glycosides
Cyanohydrin
Protocolo
Mandelonitrile is a nitrogen-containing compound, considered an essential secondary metabolite. Chemically, it is
a cyanohydrin derivative of benzaldehyde, with relevant functions in different physiological processes including
defense against phytophagous arthropods. So far, procedures for detecting mandelonitrile have been effectively
applied in cyanogenic plant species such as Prunus spp. Nevertheless, its presence in Arabidopsis thaliana,
considered a non-cyanogenic species, has never been determined. Here, we report the development of an accurate
protocol for mandelonitrile quantification in A. thaliana within the context of A. thaliana–spider mite interaction.
First, mandelonitrile was isolated from Arabidopsis rosettes using methanol; then, it was derivatized by silylation
to enhance detection and, finally, it was quantified using gas chromatography–mass spectrometry. The selectivity
and sensitivity of this method make it possible to detect low levels of mandelonitrile (LOD 3 ppm) in a plant species
considered non-cyanogenic that, therefore, will have little to no cyanogenic compounds, using a small quantity of
starting material (≥ 100 mg).
2023-06-21T11:57:21Z
2023-06-21T11:57:21Z
2023-06
info:eu-repo/semantics/article
2331-8325
http://hdl.handle.net/10259/7714
10.21769/BioProtoc.4700
eng
Bio-protocol. 2023, V. 13, n. 12, e4700
https://doi.org/10.21769/BioProtoc.4700
info:eu-repo/grantAgreement/Fundación Bancaria Caixa d'Estalvis i Pensions de Barcelona//LCF%2FPR%2FPR18%2F51130007//Fluoración Directa de Nitrocompuestos y Sales de Heteroaril Fosfonio: Síntesis de Fluorocompuestos/FluNitroPhos/
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Atribución 4.0 Internacional
Bio-protocol LLC