2026-04-23T00:03:37Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/77142023-06-22T00:05:15Zcom_10259_4313com_10259_5086com_10259_2604col_10259_4314

Isolation and Quantification of Mandelonitrile from Arabidopsis thaliana Using Gas Chromatography/Mass Spectrometry Arnáiz Alonso, Ana Vallejo García, Jorge Lucas Vallejos Calzada, Saúl Diaz, Isabel Benzaldehyde cyanohydrin Mandelonitrile Hydroxynitrile lyase Arabidopsis thaliana Spider mite Cyanogenic glycosides Cyanohydrin Química orgánica Chemistry, Organic Protocolo Mandelonitrile is a nitrogen-containing compound, considered an essential secondary metabolite. Chemically, it is a cyanohydrin derivative of benzaldehyde, with relevant functions in different physiological processes including defense against phytophagous arthropods. So far, procedures for detecting mandelonitrile have been effectively applied in cyanogenic plant species such as Prunus spp. Nevertheless, its presence in Arabidopsis thaliana, considered a non-cyanogenic species, has never been determined. Here, we report the development of an accurate protocol for mandelonitrile quantification in A. thaliana within the context of A. thaliana–spider mite interaction. First, mandelonitrile was isolated from Arabidopsis rosettes using methanol; then, it was derivatized by silylation to enhance detection and, finally, it was quantified using gas chromatography–mass spectrometry. The selectivity and sensitivity of this method make it possible to detect low levels of mandelonitrile (LOD 3 ppm) in a plant species considered non-cyanogenic that, therefore, will have little to no cyanogenic compounds, using a small quantity of starting material (≥ 100 mg). This work was supported by funds of “La Caixa” Foundation (LCF/PR18/51130007) and Ministerio de Universidades-European Union in the frame of NextGenerationEU RD 289/2021 (Universidad Politécnica de Madrid). 2023-06-21T11:57:21Z 2023-06-21T11:57:21Z 2023-06 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2331-8325 http://hdl.handle.net/10259/7714 10.21769/BioProtoc.4700 eng Bio-protocol. 2023, V. 13, n. 12, e4700 https://doi.org/10.21769/BioProtoc.4700 info:eu-repo/grantAgreement/Fundación Bancaria Caixa d'Estalvis i Pensions de Barcelona//LCF%2FPR%2FPR18%2F51130007//Fluoración Directa de Nitrocompuestos y Sales de Heteroaril Fosfonio: Síntesis de Fluorocompuestos/FluNitroPhos/ Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess application/pdf Bio-protocol LLC