2026-06-29T22:59:20Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/79222023-11-03T01:05:30Zcom_10259.4_2516com_10259_5086com_10259_2604com_10259_4313col_10259.4_2517col_10259_4314

Vallejo García, Jorge Lucas Arnáiz Alonso, Ana Busto Núñez, Mª Dolores García Pérez, José Miguel Vallejos Calzada, Saúl 2023-11-02T11:56:45Z 2023-11-02T11:56:45Z 2023-11 0014-3057 http://hdl.handle.net/10259/7922 10.1016/j.eurpolymj.2023.112495 In this study, we report the synthesis and characterization of a highly manageable polyacrylic film material for enzyme immobilization, using β-galactosidase (β-gal) as a model enzyme. The material is based on commercially available monomers and achieves efficient immobilization of β-gal through the formation of azo linkages between amino styrene groups in the polyacrylic material and the enzyme. The immobilized enzyme demonstrates superior performance compared to free enzyme in lactose hydrolysis of UHT milk, achieving lactose concentrations below 0.1% (<1 mg/mL), indicating its potential for lactose hydrolysis in dairy products. The film-shaped material is designed for easy submersion and removal, similar to a smart card, and offers reusability, with the ability to be reused at least 10 times without loss of enzymatic activity. Characterization of the immobilized enzyme on the polymeric material was performed using various techniques, including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and Raman spectroscopy. Protein release studies confirmed the stability of the immobilized enzyme during prolonged incubation in aqueous solution without significant enzyme leakage. Overall, the polyacrylic film material demonstrates promise as a simple and efficient approach for enzyme immobilization, with potential applications in various industries, including the food industry. We gratefully acknowledge the financial support provided by all funders. This work was supported by the Regional Government of Castilla y León (Junta de Castilla y León) and by the Ministry of Science and Innovation MICIN and the European Union NextGenerationEU PRTR. Author J. M. García received grant PID2020-113264RB-I00 funded by MCIN/AEI/ 10.13039/501100011033 and by “ERDF A way of making Europe”. A. Arnaiz received funding from Ministerio de Universidades-European Union in the frame of NextGenerationEU RD 289/2021 (Universidad Politécnica de Madrid). J. L. Vallejo-García received the grant PRE2021-09812 funded by MCIN/AEI/ 10.13039/501100011033 and by “ESF Investing in your future”. Author Saul Vallejos received grant BG22/00086 funded by Spanish Ministerio de Universidades. application/pdf eng Elsevier European Polymer Journal. 2023, V. 200, 112495 https://doi.org/10.1016/j.eurpolymj.2023.112495 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113264RB-I00/ES/DISEÑO, SINTESIS Y CARACTERIZACION DE POLIMEROS INTELIGENTES. APLICACION DE SENSORES QUIMICOS POLIMERICOS EN CONTROL Y SEGURIDAD ALIMENTARIA Y EN EL AMBITO SANITARIO/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica, Técnica y de Innovación 2021-2023/PRE2021-098123/ES/ info:eu-repo/grantAgreement/MIU/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/BG22%2F00086/ES/ Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess β-galactosidase Lactose-free Low-lactose Lactose hydrolysis Enzyme inmobilization Química orgánica Bioquímica Alimentos Chemistry, Organic Biochemistry Food Film-shaped reusable smart polymer to produce lactose-free milk by simple immersion info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion European Polymer Journal 200 112495