2026-06-30T04:10:01Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/79222023-11-03T01:05:30Zcom_10259.4_2516com_10259_5086com_10259_2604com_10259_4313col_10259.4_2517col_10259_4314
Repositorio Institucional de la Universidad de Burgos
author
Vallejo García, Jorge Lucas
author
Arnáiz Alonso, Ana
author
Busto Núñez, Mª Dolores
author
García Pérez, José Miguel
author
Vallejos Calzada, Saúl
2023-11-02T11:56:45Z
2023-11-02T11:56:45Z
2023-11
0014-3057
http://hdl.handle.net/10259/7922
10.1016/j.eurpolymj.2023.112495
In this study, we report the synthesis and characterization of a highly manageable polyacrylic film material for enzyme immobilization, using β-galactosidase (β-gal) as a model enzyme. The material is based on commercially available monomers and achieves efficient immobilization of β-gal through the formation of azo linkages between amino styrene groups in the polyacrylic material and the enzyme. The immobilized enzyme demonstrates superior performance compared to free enzyme in lactose hydrolysis of UHT milk, achieving lactose concentrations below 0.1% (<1 mg/mL), indicating its potential for lactose hydrolysis in dairy products. The film-shaped material is designed for easy submersion and removal, similar to a smart card, and offers reusability, with the ability to be reused at least 10 times without loss of enzymatic activity. Characterization of the immobilized enzyme on the polymeric material was performed using various techniques, including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and Raman spectroscopy. Protein release studies confirmed the stability of the immobilized enzyme during prolonged incubation in aqueous solution without significant enzyme leakage. Overall, the polyacrylic film material demonstrates promise as a simple and efficient approach for enzyme immobilization, with potential applications in various industries, including the food industry.
eng
Atribución 4.0 Internacional
β-galactosidase
Lactose-free
Low-lactose
Lactose hydrolysis
Enzyme inmobilization
Film-shaped reusable smart polymer to produce lactose-free milk by simple immersion
info:eu-repo/semantics/article
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URL
https://riubu.ubu.es/bitstream/10259/7922/1/Vallejo-epj_2023.pdf
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URL
https://riubu.ubu.es/bitstream/10259/7922/4/Vallejo-epj_2023-Supplementary_data.pdf
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Vallejo-epj_2023-Supplementary_data.pdf