2026-05-12T03:49:44Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/79222023-11-03T01:05:30Zcom_10259.4_2516com_10259_5086com_10259_2604com_10259_4313col_10259.4_2517col_10259_4314

Vallejo García, Jorge Lucas 389030ba-7531-48e5-8246-9507fadb278f 600 Arnáiz Alonso, Ana 762 600 0000-0002-3842-498X Busto Núñez, Mª Dolores 82 600 0000-0003-1647-7850 García Pérez, José Miguel 213 600 0000-0002-2674-8194 Vallejos Calzada, Saúl 567 600 0000-0001-5522-6574 2023-11-02T11:56:45Z 2023-11-02T11:56:45Z 2023-11 0014-3057 http://hdl.handle.net/10259/7922 10.1016/j.eurpolymj.2023.112495 In this study, we report the synthesis and characterization of a highly manageable polyacrylic film material for enzyme immobilization, using β-galactosidase (β-gal) as a model enzyme. The material is based on commercially available monomers and achieves efficient immobilization of β-gal through the formation of azo linkages between amino styrene groups in the polyacrylic material and the enzyme. The immobilized enzyme demonstrates superior performance compared to free enzyme in lactose hydrolysis of UHT milk, achieving lactose concentrations below 0.1% (<1 mg/mL), indicating its potential for lactose hydrolysis in dairy products. The film-shaped material is designed for easy submersion and removal, similar to a smart card, and offers reusability, with the ability to be reused at least 10 times without loss of enzymatic activity. Characterization of the immobilized enzyme on the polymeric material was performed using various techniques, including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and Raman spectroscopy. Protein release studies confirmed the stability of the immobilized enzyme during prolonged incubation in aqueous solution without significant enzyme leakage. Overall, the polyacrylic film material demonstrates promise as a simple and efficient approach for enzyme immobilization, with potential applications in various industries, including the food industry. We gratefully acknowledge the financial support provided by all funders. This work was supported by the Regional Government of Castilla y León (Junta de Castilla y León) and by the Ministry of Science and Innovation MICIN and the European Union NextGenerationEU PRTR. Author J. M. García received grant PID2020-113264RB-I00 funded by MCIN/AEI/ 10.13039/501100011033 and by “ERDF A way of making Europe”. A. Arnaiz received funding from Ministerio de Universidades-European Union in the frame of NextGenerationEU RD 289/2021 (Universidad Politécnica de Madrid). J. L. Vallejo-García received the grant PRE2021-09812 funded by MCIN/AEI/ 10.13039/501100011033 and by “ESF Investing in your future”. Author Saul Vallejos received grant BG22/00086 funded by Spanish Ministerio de Universidades. application/pdf eng Elsevier European Polymer Journal. 2023, V. 200, 112495 https://doi.org/10.1016/j.eurpolymj.2023.112495 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113264RB-I00/ES/DISEÑO, SINTESIS Y CARACTERIZACION DE POLIMEROS INTELIGENTES. APLICACION DE SENSORES QUIMICOS POLIMERICOS EN CONTROL Y SEGURIDAD ALIMENTARIA Y EN EL AMBITO SANITARIO/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica, Técnica y de Innovación 2021-2023/PRE2021-098123/ES/ info:eu-repo/grantAgreement/MIU/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/BG22%2F00086/ES/ Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess β-galactosidase Lactose-free Low-lactose Lactose hydrolysis Enzyme inmobilization Química orgánica Bioquímica Alimentos Chemistry, Organic Biochemistry Food Film-shaped reusable smart polymer to produce lactose-free milk by simple immersion info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion European Polymer Journal 200 112495 THUMBNAIL Vallejo-epj_2023.pdf.jpg Vallejo-epj_2023.pdf.jpg IM Thumbnail image/jpeg 4919 https://riubu.ubu.es/bitstream/10259/7922/5/Vallejo-epj_2023.pdf.jpg 3077eab5cdef455f8d4c730ae14b3132 MD5 5 Vallejo-epj_2023-Supplementary_data.pdf.jpg Vallejo-epj_2023-Supplementary_data.pdf.jpg IM Thumbnail image/jpeg 3922 https://riubu.ubu.es/bitstream/10259/7922/6/Vallejo-epj_2023-Supplementary_data.pdf.jpg 5f66346611736c2a4aba321b2c7d53d2 MD5 6 LICENSE license.txt license.txt text/plain; charset=utf-8 998 https://riubu.ubu.es/bitstream/10259/7922/3/license.txt aa90105e1c90a1b4fe810649aa967b7d MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 908 https://riubu.ubu.es/bitstream/10259/7922/2/license_rdf 0175ea4a2d4caec4bbcc37e300941108 MD5 2 ORIGINAL Vallejo-epj_2023.pdf Vallejo-epj_2023.pdf application/pdf 4210529 https://riubu.ubu.es/bitstream/10259/7922/1/Vallejo-epj_2023.pdf 6ef2902bb0c917c7347540df00f4df37 MD5 1 Vallejo-epj_2023-Supplementary_data.pdf Vallejo-epj_2023-Supplementary_data.pdf Supplementary data application/pdf 226530 https://riubu.ubu.es/bitstream/10259/7922/4/Vallejo-epj_2023-Supplementary_data.pdf 25d4047cd28f9768629ecb486fb9f1be MD5 4 10259/7922 oai:riubu.ubu.es:10259/7922 2023-11-03 02:05:30.081 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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