2026-04-27T17:50:28Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/80692023-11-22T01:05:28Zcom_10259_5827com_10259_5086com_10259_2604col_10259_5828

Durán Fernández-Feijóo, Cristina 8da436f4-a27b-493f-8091-16227624137c 600 Rodríguez-Fanjul, Javier 16519a93-3b22-4971-b8dd-6beaafa13a09 600 López-Abat, Miriam 1276d33f-a864-4a5c-b222-e2fef17e22f3 600 Hadley, Stephanie 99903d6e-31e2-4f49-ac7f-0bdd2aed1446 600 Cavia Saiz, Mónica 116 600 0000-0001-5132-381X Muñiz Rodríguez, Pilar 380 600 0000-0002-9306-0082 Arnaez, Juan d89bfd1f-ce6b-47e6-9ad2-dbb7090ff517 600 Fernández-Lorenzo, José Ramón 8758be6c-950d-4368-8d53-ede29fc5e824 600 Camprubí Camprubí, Marta 6fdede32-6f7f-4e6b-8420-2c56aa2cffce 600 2023-11-21T08:12:55Z 2023-11-21T08:12:55Z 2021-09 http://hdl.handle.net/10259/8069 10.3390/antiox10101523 2076-3921 Hypoxic ischemic encephalopathy (HIE) is one of the main causes of morbidity and mortality during the neonatal period, despite treatment with hypothermia. There is evidence that oxidative damage plays an important role in the pathophysiology of hypoxic-ischemic (HI) brain injury. Our aim was to investigate whether postnatal allopurinol administration in combination with hypothermia would reduce oxidative stress (OS) biomarkers in an animal model of HIE. Postnatal 10-day rat pups underwent unilateral HI of moderate severity. Pups were randomized into: Sham operated, hypoxic-ischemic (HI), HI + allopurinol (HIA), HI + hypothermia (HIH), and HI + hypothermia + allopurinol (HIHA). Biomarkers of OS and antioxidants were evaluated: GSH/GSSG ratio and carbonyl groups were tested in plasma. Total antioxidant capacity (TAC) was analyzed in plasma and cerebrospinal fluid, and 8-iso-prostaglandin F2α was measured in brain tissue. Plasma 2,20–azinobis- (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) levels were preserved in those groups that received allopurinol and dual therapy. In cerebrospinal fluid, only the HIA group presented normal ferric reducing ability of plasma (FRAP) levels. Protein oxidation and lipid peroxidation were significantly reduced in all groups treated with hypothermia and allopurinol, thus enhancing neuroprotection in HIE. application/pdf eng MDPI Antioxidants. 2021, V. 10, n. 10, 1523 https://doi.org/10.3390/antiox10101523 Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Allopurinol Hypothermia Hypoxic-ischemic encephalopathy Oxidative stress Oxidative damage Bioquímica Biología molecular Biochemistry Molecular biology Effects of Hypothermia and Allopurinol on Oxidative Status in a Rat Model of Hypoxic Ischemic Encephalopathy info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Antioxidants 10 10 THUMBNAIL Durán-antioxidants_2021.pdf.jpg Durán-antioxidants_2021.pdf.jpg IM Thumbnail image/jpeg 3537 https://riubu.ubu.es/bitstream/10259/8069/4/Dur%c3%a1n-antioxidants_2021.pdf.jpg 842893e4f2beef49f8f8cd34f4b99eda MD5 4 LICENSE license.txt license.txt text/plain; charset=utf-8 999 https://riubu.ubu.es/bitstream/10259/8069/3/license.txt b295bcbce42e2caabeb0c623d3860c06 MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 908 https://riubu.ubu.es/bitstream/10259/8069/2/license_rdf 0175ea4a2d4caec4bbcc37e300941108 MD5 2 ORIGINAL Durán-antioxidants_2021.pdf Durán-antioxidants_2021.pdf application/pdf 1692188 https://riubu.ubu.es/bitstream/10259/8069/1/Dur%c3%a1n-antioxidants_2021.pdf e652f036a91b1a0db7ac043a0b21f8e2 MD5 1 10259/8069 oai:riubu.ubu.es:10259/8069 2023-11-22 02:05:28.413 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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