2026-05-04T17:24:51Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/80722023-11-22T01:05:32Zcom_10259_5827com_10259_5086com_10259_2604col_10259_5828
Herreros-Villanueva, Marta
9af5e059-651d-4189-94e5-308e73b0282b
600
Muñiz Rodríguez, Pilar
380
600
0000-0002-9306-0082
García Girón, Carlos
a191a584-8644-4b54-b047-affd824c0493
600
Cavia Saiz, Mónica
116
600
0000-0001-5132-381X
Coma del Corral, María J.
ebb0affa-e80c-446f-90f9-bdc392d4a72c
600
2023-11-21T08:59:34Z
2023-11-21T08:59:34Z
2010-02
http://hdl.handle.net/10259/8072
10.1186/1479-5876-8-15
1479-5876
Background: Although there have been many studies on the p73 gene, some of its functions still remain unclear.
There is little research on the relationship between p73 gene transcription and its protein expression and the
response to certain drugs such as oxaliplatin and cetuximab, which are drugs currently used in colorectal cancer.
The purpose of this study was to evaluate the impact of TAp73 expression on oxaliplatin and cetuximab-based
chemotherapy in colorectal cancer cell lines with different K-Ras and B-Raf mutational status.
Methods: TAp73 was analyzed in three colorectal tumor cell lines HT-29, SW-480 and Caco-2. mRNA TAp73 was
determined using Real time PCR; TAp73 protein by immunoblotting and cell viability was analyzed by the MTT
method.
Results: We found that mRNA and TAp73 protein were decreased in cells treated with oxaliplatin (in monotherapy
or combined with cetuximab) when B-Raf is mutated. This was statistically significant and was also associated with
higher cell viability after the treatment.
Conclusions: Here, for the first time we report, that there is a signaling loop between B-Raf activation and p73
function.
Low expression of TAp73 in colorectal cancer cell lines with mutated B-Raf may be involved in the lack of response
to oxaliplatin in monotherapy or combined with cetuximab.
We thank B. De La Nogal and the Pharmacy Department for their generous help. Also, we thank CMV and her group in Leon. This work was supported by a grant FIS CA08/00070 from Instituto de Salud Carlos III, Spanish Ministerio de Ciencia e Innovación to MHV and Fundación Burgos por la Investigación de la Salud. MHV is especially thankful to CVP, IHH and AHV, for their support.
application/pdf
eng
Springer Nature
Journal of Translational Medicine. 2010, V. 8, n. 1
https://doi.org/10.1186/1479-5876-8-15
Attribution 3.0 Unported
http://creativecommons.org/licenses/by/3.0/
info:eu-repo/semantics/openAccess
Salud
Biología molecular
Health
Molecular biology
TAp73 is one of the genes responsible for the lack of response to chemotherapy depending on B-Raf mutational status
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Journal of Translational Medicine
8
1
THUMBNAIL
Herreros-jtm_2010.pdf.jpg
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Herreros-jtm_2010.pdf
Herreros-jtm_2010.pdf
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10259/8072
oai:riubu.ubu.es:10259/8072
2023-11-22 02:05:32.144
Repositorio Institucional de la Universidad de Burgos
bubrep@ubu.es
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