2026-06-29T23:01:47Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/82642024-01-10T01:05:31Zcom_10259.4_2516com_10259_5086com_10259_2604col_10259.4_2517

Mvila, Beaufray Gilaime Pilar Izquierdo, María Concepción Busto Núñez, Mª Dolores Pérez Mateos, Manuel Ortega Santamaría, Natividad 2024-01-09T12:04:02Z 2024-01-09T12:04:02Z 2015-09 0022-5142 http://hdl.handle.net/10259/8264 10.1002/jsfa.7466 1097-0010 BACKGROUND Most N fertilizers added to soil are not efficiently used by plants and are lost to the atmosphere or leached from the soil, causing environmental pollution and increasing cost. Barley seed encapsulation in calcium alginate gels containing free or immobilized urease to enhance plant utilization of soil N was investigated. RESULTS Urease was immobilized with soil humic acids (HA). A central composite face-centered design was applied to optimize the immobilization process, reaching an immobilization yield of 127%. Soil stability of urease was enhanced after the immobilization. Seed encapsulation with free urease (FU) and humic–urease complex (HUC) resulted in a urease activity retention in the coating layer of 46% and 24%, and in germination rates of 87% and 92%, respectively. Under pot culture conditions, the pots planted with seeds encapsulated with FU and HUC showed higher ammonium N (NH4+-N) (26% and 64%, respectively) than the control soil at 28 days after planting (DAP). Moreover, the seed encapsulation with FU and HUC increased the N uptake 83% and 97%, respectively, at 35 DAP. CONCLUSION Seed encapsulation with urease could substantially contribute to enhancing plant N nutrition in the early stages of seedling establishment. We thank the ‘Ministere de L’Enseignement Superieur de laRepublique du Congo’ for a fellowship awarded to BG Mvila. application/pdf eng Society of Chemical Industry Journal of the Science of Food and Agriculture. 2016, V. 96, n. 9. p. 2981-2989 https://doi.org/10.1002/jsfa.7466 Enzyme immobilization Nitrogen availability Plant growth Seed coating Seed encapsulation Soil urease Bioquímica Agricultura Biochemistry Agriculture Synthesis and characterization of a stable humic–urease complex: application to barley seed encapsulation for improving N uptake info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion info:eu-repo/semantics/openAccess Journal of the Science of Food and Agriculture 96 9 2981 2989