2026-04-23T00:07:27Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/83752024-01-18T01:05:20Zcom_10259_4862com_10259_5086com_10259_2604col_10259_4863
Shnyrova, Anna V.
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600
Ayllón Barasoain, Juan
852
600
0000-0002-7556-8106
Mikhalyov, Ilya I.
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600
Villar, Enrique
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600
Zimmerberg, Joshua
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600
Frolov, Vadim A.
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2024-01-17T12:40:53Z
2024-01-17T12:40:53Z
2007-11
0021-9525
http://hdl.handle.net/10259/8375
10.1083/jcb.200705062
1540-8140
The shape of enveloped viruses depends critically on an internal protein matrix, yet it remains unclear how the matrix proteins control the geometry of the envelope membrane. We found that matrix proteins purified from Newcastle disease virus adsorb on a phospholipid bilayer and condense into fluidlike domains that cause membrane deformation and budding of spherical vesicles, as seen by fluorescent and electron microscopy. Measurements of the electrical admittance of the membrane resolved the gradual growth and rapid closure of a bud followed by its separation to form a free vesicle. The vesicle size distribution, confined by intrinsic curvature of budding domains, but broadened by their merger, matched the virus size distribution. Thus, matrix proteins implement domain-driven mechanism of budding, which suffices to control the shape of these proteolipid vesicles.
Supported by the intramural research program of the National Institute of Child Health and Human Development; Spanish Fondo de Investigaciones Sanitarias grant FIS-PI051796, cofinanced by Fonds Européen de Développement Régional-Fonds Social Européen; and the Spanish Ministerio de Educación y Ciencia Formacíon de Profesorado Universitario program (predoctoral fellowship AP-2004-6065 to J. Ayllon).
application/pdf
eng
Rockefeller University Press
The Journal of Cell Biology. 2007, V. 179, n. 4, p. 627-633
https://doi.org/10.1083/jcb.200705062
Bioquímica
Biochemistry
Vesicle formation by self-assembly of membrane-bound matrix proteins into a fluidlike budding domain
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/openAccess
The Journal of Cell Biology
179
4
627
633
THUMBNAIL
Shnyrova-jcb_2007.pdf.jpg
Shnyrova-jcb_2007.pdf.jpg
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LICENSE
license.txt
license.txt
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https://riubu.ubu.es/bitstream/10259/8375/2/license.txt
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ORIGINAL
Shnyrova-jcb_2007.pdf
Shnyrova-jcb_2007.pdf
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https://riubu.ubu.es/bitstream/10259/8375/1/Shnyrova-jcb_2007.pdf
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10259/8375
oai:riubu.ubu.es:10259/8375
2024-01-18 02:05:20.744
Repositorio Institucional de la Universidad de Burgos
bubrep@ubu.es
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