2026-04-23T00:07:23Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/83772024-01-18T01:05:23Zcom_10259_4862com_10259_5086com_10259_2604col_10259_4863

Hai, Rong 1a9cad2d-ee8d-49b0-bed3-b83b4811d3cf 600 Martínez Sobrido, Luis f16f95c2-ef6b-4019-ae31-a423716b0eda 600 Fraser, Kathryn A. 8739a5ac-5592-44f9-9987-e752d5dea7fb 600 Ayllón Barasoain, Juan 852 600 0000-0002-7556-8106 García Sastre, Adolfo 9de2ea8b-c019-414d-bb90-b1d51ef5bbd7 600 Palese, Peter 3d436a2f-75f5-4eb2-8bf3-bb6f163e219f 600 2024-01-17T13:11:12Z 2024-01-17T13:11:12Z 2008-11 0022-538X http://hdl.handle.net/10259/8377 10.1128/jvi.01213-08 1098-5514 Type B influenza viruses can cause substantial morbidity and mortality in the population, and vaccinationremains by far the best means of protection against infections with these viruses. Here, we report theconstruction of mutant influenza B viruses for potential use as improved live-virus vaccine candidates.Employing reverse genetics, we altered the NS1 gene, which encodes a type I interferon (IFN) antagonist. Theresulting NS1 mutant viruses induced IFN and, as a consequence, were found to be attenuated in vitro and invivo. The absence of pathogenicity of the NS1 mutants in both BALB/c and C57BL/6 PKR / mice wasconfirmed. We also provide evidence that influenza B virus NS1 mutants induce a self-adjuvanted immuneresponse and confer effective protection against challenge with both homologous and heterologous B virusstrains in mice. We thank Mark A. Yondola for helpful discussions and criticalreviewing. We also express our appreciation to Christopher Narbus,Lily Ngai, and Richard Cadagan for excellent technical assistance.This work was supported by grants from the NIH, R01 AI46954 (toA.G.-S.), U01 AI70469, and U19 AI62623 (Center for InvestigatingViral Immunity and Antagonism), and by CRIP (Center for Researchon Influenza Pathogenesis, NIAID contract HHSN266200700010C).K.A.F. was supported by NIH training grant T32 AI007647. application/pdf eng American Society for Microbiology Journal of Virology. 2008, V. 82, n. 21, p. 10580-10590 https://doi.org/10.1128/jvi.01213-08 Microbiología Medicina Biología molecular Microbiology Medicine Molecular biology Influenza B Virus NS1-Truncated Mutants: Live-Attenuated Vaccine Approach info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/openAccess Journal of Virology 82 21 10580 10590 THUMBNAIL Hai-jv_2008.pdf.jpg Hai-jv_2008.pdf.jpg IM Thumbnail image/jpeg 3637 https://riubu.ubu.es/bitstream/10259/8377/3/Hai-jv_2008.pdf.jpg 31a93bf7b412103239d95b91124f9ba0 MD5 3 LICENSE license.txt license.txt text/plain; charset=utf-8 999 https://riubu.ubu.es/bitstream/10259/8377/2/license.txt b295bcbce42e2caabeb0c623d3860c06 MD5 2 ORIGINAL Hai-jv_2008.pdf Hai-jv_2008.pdf application/pdf 1985544 https://riubu.ubu.es/bitstream/10259/8377/1/Hai-jv_2008.pdf 37cbf759deaa12c61cafa34005c75e54 MD5 1 10259/8377 oai:riubu.ubu.es:10259/8377 2024-01-18 02:05:23.617 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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