2026-04-28T22:11:54Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/85962024-02-07T01:05:22Zcom_10259.4_2516com_10259_5086com_10259_2604col_10259.4_2517

García-Calvo, Laura 8e5dd201-75ef-4e51-b83d-dc8863c73176 500 Rodríguez-Castro, Raquel 19c89068-360b-4906-8d11-7d6701b16d9c 500 Ullán, Ricardo V. 4416b3bd-52ba-4474-a8c4-9be02de63c0f 500 Albillos García, Silvia María 12 600 0000-0002-3273-0126 Fernández-Aguado, Marta c1291e1c-ac26-4f5c-9cc5-8641cb271cf8 Vicente, Cláudia M. 9bea0602-b130-4f73-9244-eabf71868829 Degnes, Kristin F. 7ea802e0-dfcf-496f-91d4-0ef2f1692841 Sletta, Sletta 2570e38a-dfd0-4bfd-8211-0f87393b35c7 Barreiro, Carlos f31f8c72-7483-4d70-a29b-9bc11a4307e5 2024-02-06T09:30:59Z 2024-02-06T09:30:59Z 2023-02 0175-7598 http://hdl.handle.net/10259/8596 10.1007/s00253-022-12335-w 1432-0614 Plant biomass is a promising substrate for biorefinery, as well as a source of bioactive compounds, platform chemicals, and precursors with multiple industrial applications. These applications depend on the hydrolysis of its recalcitrant structure. However, the effective biological degradation of plant cell walls requires several enzymatic groups acting synergistically, and novel enzymes are needed in order to achieve profitable industrial hydrolysis processes. In the present work, a feruloyl esterase (FAE) activity screening of Penicillium spp. strains revealed a promising candidate (Penicillium rubens Wisconsin 54–1255; previously Penicillium chrysogenum), where two FAE-ORFs were identified and subsequently overexpressed. Enzyme extracts were analyzed, confirming the presence of FAE activity in the respective gene products (PrFaeA and PrFaeB). PrFaeB-enriched enzyme extracts were used to determine the FAE activity optima (pH 5.0 and 50–55 °C) and perform proteome analysis by means of MALDI-TOF/TOF mass spectrometry. The studies were completed with the determination of other lignocellulolytic activities, an untargeted metabolite analysis, and upscaled FAE production in stirred tank reactors. The findings described in this work present P. rubens as a promising lignocellulolytic enzyme producer. This study was funded by a project (ref. no. CTM2012-32026) of the Spanish Ministry of Economy, Industry and Competitiveness (MINECO) and co-funded by the ERDF (European Regional Development Fund). Ms. Laura García-Calvo was supported by a PhD grant (ref. no. BES-2013–064578) and an international stay grant (ref. no. EEBB-I-16–11760), awarded by the Spanish Ministry MINECO. application/pdf eng Springer Applied Microbiology and Biotechnology. 2023, V. 107, n. 2-3, p. 691–717 https://doi.org/10.1007/s00253-022-12335-w Fungi Penicillium rubens Penicillium chrysogenum Lignocellulose Feruloyl esterase Extracellular proteome Bioquímica Biología molecular Biochemistry Molecular biology Penicillium chrysogenum as a fungal factory for feruloyl esterases info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion info:eu-repo/semantics/openAccess Applied Microbiology and Biotechnology 107 2-3 691 717 THUMBNAIL Garcia-amb_2023.pdf.jpg Garcia-amb_2023.pdf.jpg IM Thumbnail image/jpeg 4589 https://riubu.ubu.es/bitstream/10259/8596/3/Garcia-amb_2023.pdf.jpg 6e0d8f41036dd291d1ad5198bc9556e5 MD5 3 LICENSE license.txt license.txt text/plain; charset=utf-8 1362 https://riubu.ubu.es/bitstream/10259/8596/2/license.txt 5d013bfa6e473ff0db22cd82a4d71a70 MD5 2 ORIGINAL Garcia-amb_2023.pdf Garcia-amb_2023.pdf application/pdf 2691600 https://riubu.ubu.es/bitstream/10259/8596/1/Garcia-amb_2023.pdf bb9e4083003fa18ba6821399a1819de8 MD5 1 10259/8596 oai:riubu.ubu.es:10259/8596 2024-02-07 02:05:22.385 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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