2026-04-29T18:20:57Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/86322025-01-23T01:05:15Zcom_10259_9895com_10259_3989com_10259.4_106com_10259_2604col_10259_9896

Pérez Fernández, Cristian 41151d83-48a8-4363-a23a-b2cf33d3d481 600 Morales Navas, Miguel 872 500 0000-0001-7222-2743 Guardia Escote, Laia c642b9b9-7d83-4118-b8c5-f018fa660d79 600 Garrido Cárdenas, José Antonio fcd94710-d219-4c38-9d4a-242c7ad1f982 600 Colomina, María Teresa b33a2d2e-b902-4a99-809f-551a9af83d1c 600 Giménez, Estela ef5db8db-2f1a-4c20-a3c1-c79a073c5b22 600 Sánchez Santed, Fernando 3945434c-d67b-4d35-8c6d-262ce027f039 600 2024-02-08T11:31:10Z 2024-02-08T11:31:10Z 2020-01 0278-6915 http://hdl.handle.net/10259/8632 10.1016/j.fct.2019.110865 Development is especially sensitive to Chlorpyrifos (CPF) toxicity, associated with several neurodegenerative and neurodevelopmental disorders where motor function dysfunction is a core symptom. Amongst the alternative molecular targets to cholinesterases inhibition, developmental CPF alters different components in the most important neurotransmitter systems, although this depends on the exposure period. Exposure during the late postnatal preweaning stage is the least studied by far. This period includes essential neurodevelopmental processes and has an important translational meaning. The present study analyzed the influence of low doses of CPF on this developmental window on locomotor activity and the state of the different neurotransmitter systems by pharmacological challenges. Brain gene expression and microbiome modulation following CPF were also analyzed. CPF exposure long-term increased spontaneous vertical activity, female's activity following acute stress, hyposensitized the cholinergic system and hypersensitized the GABAergic system, up-regulated both muscarinic 2 receptor and GABA-A-α2 receptor subunit in the dorsal striatum and the frontal cortex, respectively and induced gut microbiota dysbiosis at both genus and species levels. The present study supports alternative molecular targets than the ChEs following late postnatal, preweaning exposure to low doses of CPF, focusing on both cholinergic and GABAergic systems and the gut microbiome as an important factor. This study was supported by two grants from the Ministerio de Economía y Competitividad, Spanish Government and the Fondo Europeo de Desarrollo Regional (MINECO-FEDER). Grant numbers: PSI2017-86847-C2-1-R MINECO-FEDER and PSI2017-86847-C2-2-R. application/pdf eng Elsevier Food and Chemical Toxicology. 2020, V. 135, 110865 https://doi.org/10.1016/j.fct.2019.110865 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Chlorpyrifos Locomotor Cholinergic system GABAergic system Gene expression Gut microbiome Fisiología Biología molecular Physiology Molecular biology Long-term effects of low doses of Chlorpyrifos exposure at the preweaning developmental stage: A locomotor, pharmacological, brain gene expression and gut microbiome analysis info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Food and Chemical Toxicology 135 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 805 https://riubu.ubu.es/bitstream/10259/8632/5/license_rdf 4460e5956bc1d1639be9ae6146a50347 MD5 5 THUMBNAIL Perez-fct_2020.pdf.jpg Perez-fct_2020.pdf.jpg IM Thumbnail image/jpeg 3559 https://riubu.ubu.es/bitstream/10259/8632/3/Perez-fct_2020.pdf.jpg 55d5f551c69b75ea78adb44d7c7c6f40 MD5 3 Perez-fct__2020.pdf.jpg Perez-fct__2020.pdf.jpg IM Thumbnail image/jpeg 3827 https://riubu.ubu.es/bitstream/10259/8632/6/Perez-fct__2020.pdf.jpg c73da5b5c247a5d08cd3e5db0cd19fd8 MD5 6 LICENSE license.txt license.txt text/plain; charset=utf-8 999 https://riubu.ubu.es/bitstream/10259/8632/2/license.txt b295bcbce42e2caabeb0c623d3860c06 MD5 2 ORIGINAL Perez-fct__2020.pdf Perez-fct__2020.pdf application/pdf 658803 https://riubu.ubu.es/bitstream/10259/8632/4/Perez-fct__2020.pdf ece28055bdd46f117484e55e66faa3ac MD5 4 10259/8632 oai:riubu.ubu.es:10259/8632 2025-01-23 02:05:15.768 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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