2026-04-23T00:08:28Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/91922024-05-28T00:05:13Zcom_10259_3592com_10259_3591com_10259.4_106com_10259_2604com_10259_4313com_10259_5086col_10259_9193col_10259_7330
Vallejo García, Jorge Lucas
864
600
Cutillo Foraster, Alessandra
95a27f3b-0b2a-4b13-8951-452f458dc5b7
600
Arnáiz Alonso, Ana
762
600
0000-0002-3842-498X
Vallejos Calzada, Saúl
567
600
0000-0001-5522-6574
García Pérez, José Miguel
213
600
0000-0002-2674-8194
Muñoz Santamaría, María Asunción
385
600
0000-0002-0063-3057
Trigo López, Miriam
556
600
0000-0001-5948-1230
2024-05-27T12:16:58Z
2024-05-27T12:16:58Z
2024-05-22
http://hdl.handle.net/10259/9192
10.5772/intechopen.1005087
This chapter explores lactose hydrolysis, emphasizing conventional techniques
and the noteworthy immobilization of β-galactosidase on polymeric matrices to
enhance the process. Lactose, present in milk and dairy, poses challenges for lactoseintolerant
individuals, requiring enzymatic hydrolysis for lactose-free product development.
The presence of other milk components, such as proteins and minerals, can
indirectly influence the efficiency of lactose hydrolysis by potentially interacting with
β-galactosidase enzyme or affecting its stability and activity, making it necessary to
control factors such as enzyme concentration, temperature, pH, and reaction time to
improve lactose hydrolysis rates. The chapter delves into established methodologies,
covering enzymatic kinetics, reaction conditions, and substrate concentrations. It
also describes the innovative approach of immobilizing β-galactosidase on polymeric
supports to enhance enzyme stability, reusability, and overall efficiency in lactose
hydrolysis. Discussions include the design of suitable polymeric matrices, providing
insights into mechanisms governing catalytic performance. This comprehensive
exploration contributes to understanding lactose hydrolysis, offering valuable insights
for developing efficient and sustainable enzymatic processes applicable to the food
and pharmaceutical industries.
We gratefully acknowledge the financial support provided by all funders. This work was supported by the Regional Government of Castilla y León (Junta de Castilla y León) and by the Ministry of Science and Innovation MICIN and the European Union NextGenerationEU PRTR. Author Saul Vallejos received grant BG22/00086 funded by the Spanish Ministerio de Universidades. Author Jose Miguel García received grant PID2020-113264RB-I00 funded by MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe,” The financial support provided by Fondo Europeo de Desarrollo Regional-European Regional Development Fund (FEDER, ERDF) and Regional Government of Castilla y León -Consejería de Educación, Junta de Castilla y León- (BU025P23) is gratefully acknowledged.
eng
IntechOpen
Milk Proteins - Technological Innovations, Nutrition, Sustainability and Novel Applications
https://www.intechopen.com/online-first/1188731#
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Diary products
Lactose hydrolysis
Enzyme immobilization
Advanced polymers
Polymeric supports
Química orgánica
Alimentos
Polímeros
Chemistry, Organic
Food
Polymers
Hydrolysis of Lactose: Conventional Techniques and Enzyme Immobilization Strategies on Polymeric Supports
info:eu-repo/semantics/bookPart
info:eu-repo/semantics/publishedVersion
THUMBNAIL
Vallejo-Hydrolysis_Lactose_2024.pdf.jpg
Vallejo-Hydrolysis_Lactose_2024.pdf.jpg
IM Thumbnail
image/jpeg
2378
https://riubu.ubu.es/bitstream/10259/9192/4/Vallejo-Hydrolysis_Lactose_2024.pdf.jpg
6df2acc8756947e6bab818621f9d89a0
MD5
4
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1362
https://riubu.ubu.es/bitstream/10259/9192/3/license.txt
5d013bfa6e473ff0db22cd82a4d71a70
MD5
3
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
908
https://riubu.ubu.es/bitstream/10259/9192/2/license_rdf
0175ea4a2d4caec4bbcc37e300941108
MD5
2
ORIGINAL
Vallejo-Hydrolysis_Lactose_2024.pdf
Vallejo-Hydrolysis_Lactose_2024.pdf
application/pdf
2273560
https://riubu.ubu.es/bitstream/10259/9192/1/Vallejo-Hydrolysis_Lactose_2024.pdf
717509081fad974813a227ec193e4d3c
MD5
1
10259/9192
oai:riubu.ubu.es:10259/9192
2024-05-28 02:05:13.961
Repositorio Institucional de la Universidad de Burgos
bubrep@ubu.es
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