2026-04-17T04:59:09Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/95002024-07-27T00:05:34Zcom_10259_4323com_10259_5086com_10259_2604com_10259_9491com_10259_6168col_10259_4330col_10259_9492col_10259_6169

00925njm 22002777a 4500 dc Parra de la Parra, Sandra de la author Fernández Pampín, Natalia author Garroni, Sebastiano author Poddighe, Matteo author Fuente Vivas, Dalia de la author Barros García, Rocío author Martel Martín, Sonia author Aparicio Martínez, Santiago author Rumbo Lorenzo, Carlos author Tamayo Ramos, Juan Antonio author 2024-05 Despite the wide application of graphene-based materials, the information of the toxicity associated to some specific derivatives such as aminated graphene oxide is scarce. Likewise, most of these studies analyse the pristine materials, while the available data regarding the harmful effects of degraded forms is very limited. In this work, the toxicity of graphene oxide (GO), aminated graphene oxide (GO-NH2), and their respective degraded forms (dGO and dGO-NH2) obtained after being submitted to high-intensity sonication was evaluated applying in vitro assays in different models of human exposure. Viability and ROS assays were performed on A549 and HT29 cells, while their skin irritation potential was tested on a reconstructed human epidermis model. The obtained results showed that GO-NH2 and dGO-NH2 substantially decrease cell viability in the lung and gastrointestinal models, being this reduction slightly higher in the cells exposed to the degraded forms. In contrast, this parameter was not affected by GO and dGO which, conversely, showed the ability to induce higher levels of ROS than the pristine and degraded aminated forms. Furthermore, none of the materials is skin irritant. Altogether, these results provide new insights about the potential harmful effects of the selected graphene-based nanomaterials in comparison with their degraded counterparts. 0300-483X http://hdl.handle.net/10259/9500 10.1016/j.tox.2024.153783 A549 cells HT29 cells 3D RhE model Physical degradation Comparative toxicological analysis of two pristine carbon nanomaterials (graphene oxide and aminated graphene oxide) and their corresponding degraded forms using human in vitro models