2026-04-17T05:00:08Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/95002024-07-27T00:05:34Zcom_10259_4323com_10259_5086com_10259_2604com_10259_9491com_10259_6168col_10259_4330col_10259_9492col_10259_6169

Parra de la Parra, Sandra de la 838 600 Fernández Pampín, Natalia 810 600 Garroni, Sebastiano 227 600 0000-0001-7686-6589 Poddighe, Matteo c45f82ea-c812-4f84-927c-e9c3f3f89dfa 600 Fuente Vivas, Dalia de la 5da65f32-c20e-4d52-aae2-d47bc89b40ba 600 Barros García, Rocío 787 600 0000-0002-0392-8504 Martel Martín, Sonia 790 600 0000-0001-9080-0877 Aparicio Martínez, Santiago 30 600 0000-0001-9996-2426 Rumbo Lorenzo, Carlos 505 600 0000-0002-5038-0334 Tamayo Ramos, Juan Antonio 547 600 0000-0002-7071-002X 2024-07-26T10:53:21Z 2024-07-26T10:53:21Z 2024-05 0300-483X http://hdl.handle.net/10259/9500 10.1016/j.tox.2024.153783 Despite the wide application of graphene-based materials, the information of the toxicity associated to some specific derivatives such as aminated graphene oxide is scarce. Likewise, most of these studies analyse the pristine materials, while the available data regarding the harmful effects of degraded forms is very limited. In this work, the toxicity of graphene oxide (GO), aminated graphene oxide (GO-NH2), and their respective degraded forms (dGO and dGO-NH2) obtained after being submitted to high-intensity sonication was evaluated applying in vitro assays in different models of human exposure. Viability and ROS assays were performed on A549 and HT29 cells, while their skin irritation potential was tested on a reconstructed human epidermis model. The obtained results showed that GO-NH2 and dGO-NH2 substantially decrease cell viability in the lung and gastrointestinal models, being this reduction slightly higher in the cells exposed to the degraded forms. In contrast, this parameter was not affected by GO and dGO which, conversely, showed the ability to induce higher levels of ROS than the pristine and degraded aminated forms. Furthermore, none of the materials is skin irritant. Altogether, these results provide new insights about the potential harmful effects of the selected graphene-based nanomaterials in comparison with their degraded counterparts. This work was supported by the Junta de Castilla y Leon-FEDER grant Nº BU058P20 (NANOCOMP), and by the European Union’s H2020 research and innovation programme, under the grant agreement No 953152 (DIAGONAL). The scholarship of Matteo Poddighe received financial support within the activities of the PhD program in Chemical and Technological Sciences. We thank Graphenea for kindly providing us with the pristine GO and GO-NH2 nanomaterials. application/pdf eng Elsevier Toxicology. 2024, V. 504, 153783 https://doi.org/10.1016/j.tox.2024.153783 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess A549 cells HT29 cells 3D RhE model Physical degradation Química analítica Química física Chemistry, Analytic Chemistry, Physical and theoretical Comparative toxicological analysis of two pristine carbon nanomaterials (graphene oxide and aminated graphene oxide) and their corresponding degraded forms using human in vitro models info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Toxicology 504 THUMBNAIL Parra-Toxicology_2024.pdf.jpg Parra-Toxicology_2024.pdf.jpg IM Thumbnail image/jpeg 4915 https://riubu.ubu.es/bitstream/10259/9500/4/Parra-Toxicology_2024.pdf.jpg 74628b05c12203e99c160ef7d97d8572 MD5 4 LICENSE license.txt license.txt text/plain; charset=utf-8 1362 https://riubu.ubu.es/bitstream/10259/9500/3/license.txt 5d013bfa6e473ff0db22cd82a4d71a70 MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 805 https://riubu.ubu.es/bitstream/10259/9500/2/license_rdf 4460e5956bc1d1639be9ae6146a50347 MD5 2 ORIGINAL Parra-Toxicology_2024.pdf Parra-Toxicology_2024.pdf application/pdf 2718651 https://riubu.ubu.es/bitstream/10259/9500/1/Parra-Toxicology_2024.pdf f98c9931799decfdb2b16e00aa03752a MD5 1 10259/9500 oai:riubu.ubu.es:10259/9500 2024-07-27 02:05:34.691 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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