2026-04-22T17:39:47Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/95832024-10-03T00:05:21Zcom_10259_4244com_10259_5086com_10259_2604col_10259_4245

Green fractionation and hydrolysis of fish meal to improve their techno-functional properties Barea Gómez, Pedro Melgosa Gómez, Rodrigo Benito Román, Oscar Illera Gigante, Alba Ester Beltrán Calvo, Sagrario Sanz Díez, Mª Teresa Fish meal protein Water fractionation Hydrolysis Subcritical water Emulsification Ingeniería química Alimentos Biotecnología Chemical engineering Food Biotechnology A green strategy employing water as solvent has been adopted to obtain protein hydrolysates from fish meal (FM), its water-soluble fraction (WSP), and its non-water-soluble fraction (NSP). The techno-functional properties of the hydrolysates have been investigated and compared to hydrolysates obtained with Alcalase®. In general, SWH hydrolysates presented higher content of free amino acids and higher degree of hydrolysis, which reflected on the molecular size distribution. However, Alcalase® hydrolysates presented better solubility (from 74 ± 4% for NSP at pH = 2 up to 99 ± 1% for WSP at pH = 4–7). According to fluorescence experiments, FM and NSP hydrolysates showed the highest surface hydrophobicity, which has been related to better emulsifying properties and higher emulsion stability. The emulsions stabilized with 2%wt. of SWH-treated NSP showed the smallest particle sizes, with D[4,3] = 155 nm at day 0, and good stability, with D[4,3] = 220 nm at day 7, proving that water fractionation followed by SWH treatment is a good method to improve the techno-functional properties of the hydrolysates. The authors acknowledge Sarval Bio-Industries Noroeste, S.A.U. (A Coruña, Spain) for kindly providing the fish meal used in this work and Novozymes A/S for kindly providing the enzyme Alcalase®. This work was supported by the Agencia Estatal de Investigación (AEI) [grant numbers PID2019-104950RB-I00 and PID2020-116716RJ-I00 ] by the AEI, Ministerio de Ciencia e Innovación (MICINN) and Next GenerationEU, [grant numbers TED2021-129311B-I00 and PDC2022-133443-I00 ] and the Junta de Castilla y León (JCyL) and the European Regional Development Fund (ERDF) [grant number BU050P20 ]. PB pre-doctoral contract is funded by JCyL and the European Social Fund (ESF) [ ORDEN EDU/1868/2022 ]. RM contract is funded by a Beatriz Galindo Research Fellowship [ BG20/00182 ]. Illera's post-doctoral contract is funded by AEI, MICINN and EU through project TED2021-129311B-I00 . OBR post-doctoral contract was funded by AEI through project PID2020–116716RJ-I00 . 2024-10-02T09:25:34Z 2024-10-02T09:25:34Z 2024-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 0308-8146 http://hdl.handle.net/10259/9583 10.1016/j.foodchem.2024.139550 eng Food Chemistry. 2024, V. 452, 139550 https://doi.org/10.1016/j.foodchem.2024.139550 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104950RB-I00/ES/VALORIZACION DE SUBPRODUCTOS MARINOS MEDIANTE TECNOLOGIAS DE FLUIDOS SUB- Y SUPERCRITICOS PARA LA OBTENCION DE BIOCOMPUESTOS VALIOSOS/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-116716RJ-I00/ES/TECNOLOGIAS DE FLUIDOS PRESURIZADOS PARA LA VALORIZACION DE SUBPRODUCTOS DE LA INDUSTRIA AGROALIMENTARIA: RECUPERACION DE PECTINA Y OTROS COMPUESTOS BIOACTIVOS DE ALTO VALOR/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica, Técnica y de Innovación 2021-2023/TED2021-129311B-I00/ES/Tecnologías verdes basadas en el empleo de fluidos presurizados para valorizar biomasa lignocelulósica mediante la producción de furfural/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica, Técnica y de Innovación 2021-2023/PDC2022-133443-I00/ES/Diseño de un prototipo para la extracción de bioproductos con agua subcrítica/ info:eu-repo/grantAgreement/Junta de Castilla y León//BU050P20//Valorización de la fracción proteica de subproductos de la industria agroalimentaria de Castilla y León mediante tecnologías de fluidos presurizados/ info:eu-repo/grantAgreement/MIU/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/BG20%2F00182/ES/ Atribución-NoComercial 4.0 Internacional http://creativecommons.org/licenses/by-nc/4.0/ info:eu-repo/semantics/openAccess application/pdf Elsevier