2026-04-17T13:52:57Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/97472024-11-30T01:05:14Zcom_10259_4313com_10259_5086com_10259_2604col_10259_4314
2024-11-29T10:56:01Z
urn:hdl:10259/9747
Naked-eye detection of Legionella pneumophila using smart fluorogenic polymers prepared as hydrophilic films, coatings, and electrospun nanofibers
Arnáiz Alonso, Ana
Guembe García, Marta
Martínez Yubero, Mario Andrés
Trigo López, Miriam
González, Edurne
Otsuka, Issei
Vallejos Calzada, Saúl
Polyacrylic films
Linear polymer
Electrospun nanofibers
Fluorescence
Legionella
Smart material
Polymeric sensors
Legionella pneumophila is a significant public health threat, responsible for severe diseases such as Legionnaires’ disease. Traditional detection methods are often labour-intensive, time-consuming, and require sophisticated equipment. This study introduces smart fluorogenic polymeric materials for the naked-eye detection of L. pneumophila via protease activity. These materials, prepared as hydrophilic films, cellulose-coated linear copolymers, and electrospun nanofibers, operate on an OFF/ON FRET system, emitting fluorescence under UV light upon interaction with L. pneumophila proteases. Characterisation confirmed the successful immobilisation of the peptide substrate and its response to proteases. The sensors showed moderate to high sensitivity and specificity, with detection limits of 2.91 × 105, 3.64 × 10⁵, and 4.04 × 105 CFUs/mL for the film, copolymer, and nanofiber formats, respectively. Cross-reactivity tests identified only Pseudomonas aeruginosa as an interferent. This novel approach offers rapid, simple, and cost-effective L. pneumophila detection with visible results under UV light, suitable for clinical and environmental samples. It highlights the potential for broader pathogen detection applications.
2024-11-29T10:56:01Z
2024-11-29T10:56:01Z
2025-02
info:eu-repo/semantics/article
0925-4005
http://hdl.handle.net/10259/9747
10.1016/j.snb.2024.136976
eng
Sensors and Actuators B: Chemical. 2025, V. 425, 136976
https://doi.org/10.1016/j.snb.2024.136976
info:eu-repo/grantAgreement/Junta de Castilla y León//BU025P23//Polímeros inteligentes. Aplicación en seguridad y control alimentario y biomédico, construcción sostenible y biorremediación (pin)/
info:eu-repo/grantAgreement/MIU/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/BG22%2F00086/ES/
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113264RB-I00/ES/DISEÑO, SINTESIS Y CARACTERIZACION DE POLIMEROS INTELIGENTES. APLICACION DE SENSORES QUIMICOS POLIMERICOS EN CONTROL Y SEGURIDAD ALIMENTARIA Y EN EL AMBITO SANITARIO/
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-117628RJ-I00/ES/FABRICACION DE NANOFIBRAS FUCIONALES UNICAS Y AVANZADAS MEDIANTE PROCESOS DE ELECTROHILADO VERDE/
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Atribución 4.0 Internacional
Elsevier