2026-04-17T13:34:30Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/97472024-11-30T01:05:14Zcom_10259_4313com_10259_5086com_10259_2604col_10259_4314

00925njm 22002777a 4500 dc Arnáiz Alonso, Ana author Guembe García, Marta author Martínez Yubero, Mario Andrés author Trigo López, Miriam author González, Edurne author Otsuka, Issei author Vallejos Calzada, Saúl author 2025-02 Legionella pneumophila is a significant public health threat, responsible for severe diseases such as Legionnaires’ disease. Traditional detection methods are often labour-intensive, time-consuming, and require sophisticated equipment. This study introduces smart fluorogenic polymeric materials for the naked-eye detection of L. pneumophila via protease activity. These materials, prepared as hydrophilic films, cellulose-coated linear copolymers, and electrospun nanofibers, operate on an OFF/ON FRET system, emitting fluorescence under UV light upon interaction with L. pneumophila proteases. Characterisation confirmed the successful immobilisation of the peptide substrate and its response to proteases. The sensors showed moderate to high sensitivity and specificity, with detection limits of 2.91 × 105, 3.64 × 10⁵, and 4.04 × 105 CFUs/mL for the film, copolymer, and nanofiber formats, respectively. Cross-reactivity tests identified only Pseudomonas aeruginosa as an interferent. This novel approach offers rapid, simple, and cost-effective L. pneumophila detection with visible results under UV light, suitable for clinical and environmental samples. It highlights the potential for broader pathogen detection applications. 0925-4005 http://hdl.handle.net/10259/9747 10.1016/j.snb.2024.136976 Polyacrylic films Linear polymer Electrospun nanofibers Fluorescence Legionella Smart material Polymeric sensors Naked-eye detection of Legionella pneumophila using smart fluorogenic polymers prepared as hydrophilic films, coatings, and electrospun nanofibers