2026-04-20T05:14:39Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/97862024-12-17T01:05:25Zcom_10259_9301com_10259_9300com_10259.4_106com_10259_2604col_10259_9302
Nascimento, Antonia Maiara Marques do
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600
Rego, Mailson M. do
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600
Souza, Bruna de B.
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600
Nascimento, Kaline da S.
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600
Pessoa, Angela M. dos S.
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Barroso, Priscila Alves
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Rego, Elizanilda R. do
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2024-12-16T09:26:16Z
2024-12-16T09:26:16Z
2025-02
1415-4366
http://hdl.handle.net/10259/9786
10.1590/1807-1929/agriambi.v29n2e279742
1807-1929
Kalanchoe daigremontiana is an ornamental species propagated exclusively asexually. However, no in vitro studies have been conducted to assess the relationship between changes in the culture environment and genetic variations of K. daigremontiana. Therefore, this study aimed to determine the optimal concentration of salts in the Murashige & Skoog (MS) medium and the optimal concentration of sucrose in the culture medium, in addition to the quality of light, in the development of in vitro plantlets from leaf explants of K. daigremontiana. Additionally, a genetic evaluation of the resulting plantlets was conducted to study phenotypic changes that could be attributed to somaclonal variation using Random Amplified Polymorphic DNA (RAPD) markers. For this purpose, leaf explants of K. daigremontiana were disinfected and inoculated in different salt concentrations (25, 50, and 100%) of MS medium without sucrose. The cultures were transferred to a growth chamber and subjected to three light conditions (white, blue, and red) with a 16-hour photoperiod, plus the absence of light, at a temperature of 27 ± 1 °C. Explants were also inoculated in 50% salt concentrations of MS medium supplemented with various concentrations of sucrose (control - without sucrose, 1.5, 3.0, 4.5, and 6.0%). K. daigremontiana leaf explants showed optimal development under white light and in any MS medium salt concentrations. A sucrose-free culture medium was viable, and no genetic variation was observed in the plantlets compared to the parent plants under the tested conditions.
eng
Universidade Federal de Campina Grande. Departamento de Engenharia Agrícola
Revista Brasileira de Engenharia Agrícola e Ambiental. 2025, V. 29, n. 2
https://doi.org/10.1590/1807-1929/agriambi.v29n2e279742
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
In vitro propagation
Ornamental plants
RAPD markers
Biotecnología agraria
Agricultural biotechnology
Ingeniería Agrícola
Agricultural engineering
Micropropagation and genetic uniformity of Kalanchoe daigremontiana (Crassulaceae)
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Revista Brasileira de Engenharia Agrícola e Ambiental
29
2
THUMBNAIL
Nascimento-rbeaa_2025.pdf.jpg
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license.txt
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Nascimento-rbeaa_2025.pdf
Nascimento-rbeaa_2025.pdf
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https://riubu.ubu.es/bitstream/10259/9786/1/Nascimento-rbeaa_2025.pdf
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10259/9786
oai:riubu.ubu.es:10259/9786
2024-12-17 02:05:25.431
Repositorio Institucional de la Universidad de Burgos
bubrep@ubu.es
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