2026-04-17T20:35:17Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/97892024-12-17T01:05:26Zcom_10259_9301com_10259_9300com_10259.4_106com_10259_2604col_10259_9302

Nascimento, Antonia Maiara Marques do Barroso, Priscila Alves Nascimento, Naysa Flavia Ferreira do Goicoa, Tomás Ugarte, María Dolores Montalbán, Itziar Aurora Moncaleán, Paloma 2024-12-16T12:24:20Z 2024-12-16T12:24:20Z 2020-11 http://hdl.handle.net/10259/9789 10.3390/f11111181 1999-4907 Climatic variations in the current environmental scenario require plants with tolerance to sudden changes in temperature and a decrease in water availability. Accordingly, this tolerance will enable successful plantations and the maintenance of natural and planted forests. Consequently, in the last two decades, drought tolerance and high temperatures in conifers have been an important target for morphological, physiological, and epigenetic studies. Based on this, our research team has optimized different stages of somatic embryogenesis (SE) in Pinus spp. improving the success of the process. Through this method, we can obtain a large amount of clonal material and then analyze the somatic plants under different conditions ex vitro. The analysis of the morphological and physiological parameters in somatic embryos (ses) and plants with different tolerances to abiotic stress can provide us with valuable information about the mechanisms used by plants to survive under adverse environmental conditions. Thus, the objective of this work was to evaluate the influence of high temperatures (23, 40, 50, and 60 °C, after 12 weeks, 90, 30, 5 min, respectively) on the morphology of somatic embryos obtained from Pinus radiata D.Don (Radiata pine) and Pinus halepensis Mill. (Aleppo pine). In addition, we carried out a physiological evaluation of the somatic plants of P. radiata submitted to heat and water stress in a greenhouse. We observed that the number of somatic embryos was not affected by maturation temperatures in both species. Likewise, P. radiata plants obtained from these somatic embryos survived drought and heat stress in the greenhouse. In addition, plants originating from embryonal masses (EMs) subjected to high maturation temperature (40 and 60 °C) had a significant increase in gs and E. Therefore, it is possible to modulate the characteristics of somatic plants produced by the manipulation of environmental conditions during the process of SE. This research was funded by MINECO (Spanish Government) project (AGL2016-76143-C4-3R), CYTED (P117RT0522), and MINECO (BES-2017-081249, “Ayudas para contratos predoctorales para la formación de doctores”). MULTIFOREVER (Project MULTIFOREVER) is supported under the umbrella of ERA-NET cofund Forest Value by ANR (FR), FNR (DE), MINCyT (AR), MINECO-AEI (ES), MMM (FI), and VINNOVA (SE). Forest value has received funding from the European Union’s Horizon 2020 research and innovation programmed under agreement No 773324. application/pdf eng MDPI Forests. 2020, V. 11, n. 11, p. 1181 https://doi.org/10.3390/f11111181 Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Abiotic stress Embryonal masses Pinus halepensis Pinus radiata Somatic embryogenesis Somatic plantlets Tolerance Biotecnología agraria Química Agricultural biotechnology Chemistry Pinus spp. Somatic Embryo Conversion under High Temperature: Effect on the Morphological and Physiological Characteristics of Plantlets info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Forests 11 11 1181