2026-04-20T09:44:21Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/98062025-03-18T23:42:10Zcom_10259_9301com_10259_9300com_10259.4_106com_10259_2604col_10259_9302

Montalbán, Itziar Aurora b4d2267d-2943-4702-a7ec-4eda18be115f 600 Castander-Olarieta, Ander 7c865706-a888-4ac2-ac49-5eb7a25e31c7 600 Nascimento, Antonia Maiara Marques do 927 500 Suárez Álvarez, Sonia 5971d8f3-77e8-476a-b526-430f74b90004 600 Polesi, Luiza Giacomolli cce0c3d6-0d79-4b9a-8610-734399dba69b 600 Back, Franklin Panato 355c9a02-11e6-42cc-8aed-1e7941dde687 600 Steiner, Neusa ad078ee1-48bd-4494-b688-ca06388397da 600 Guerra, Miguel Pedro 5908b517-75ad-433d-b9e0-610a8e8dc628 600 Moncaleán, Paloma e9220863-e037-4cf1-af9d-db10376617d6 600 2024-12-18T11:53:27Z 2024-12-18T11:53:27Z 2024-03 0167-6857 http://hdl.handle.net/10259/9806 10.1007/s11240-024-02721-9 1573-5044 Applying stress factors such as high temperatures during the different stages of somatic embryogenesis is important to uncover the molecular mechanisms of stress response and adaptation, and as a strategy to produce plants adapted to harsh environmental conditions derived from climate changes. In this sense, the present work aims to study the effect of high temperatures applied during the maturation of somatic embryogenesis in the ploidy stability, the amino acid and polyamine profiles of the somatic embryos obtained, and the morphological characteristics of the somatic plantlets. The results revealed that the maturation temperature did not affect the morphology of the resulting somatic plantlets, nor the ploidy and genome size of phenotypically normal somatic embryos, whose ploidy and DNA content levels were similar to those found in mature zygotic embryos. Nonetheless, a slight but significant reduction of the genome size of aberrant somatic embryos was observed. The maturation temperature changed the levels of glycine, arginine, lysine, and ornithine. These last three amino acids are precursors of the polyamines detected. Regarding this, putrescine levels were higher in somatic embryos from the highest maturation temperature (5 min pulse at 60 ºC), however, the amount of this polyamine in all samples was much lower than spermidine, spermine and cadaverine. In conclusion, the different temperatures applied did not lead to substantial changes in the ploidy level, endogenous PAs of the somatic embryos developed, or in the morphology of the somatic plantlets. Significant changes in the endogenous amino acids were observed, which may be linked to PAs metabolism and other metabolic pathways involved in stress response. This research was funded by MICINN project (AGL2016- 76143-C4-3R and PID2020-112627RB-C32), CYTED (P117RT0522), DECO (Basque government, AMM PhD fellowship), COST Action CA21157 “European Network for Innovative Woody Plant Cloning”, www.copytree.eu, supported by COST (European Cooperation in Science and Technology) www.cost.eu, and MULTIFOREVER project, supported under the umbrella of ERA-NET Cofund ForestValue by ANR(FR), FNR (DE), MINCyT (AR), MINECO-AEI (ES), MMM (FI), and VINNOVA (SE). ForestValue has received funding from the European Union’sHorizon 2020 Research and Innovation Programme under grant agreement no. 773324. MPG received funds from CNPq/Brazil (Proc. 302798/2018-8, and 407974/2018-0) application/pdf eng Springer Plant Cell, Tissue and Organ Culture. 2024, V. 157, n. 3 https://doi.org/10.1007/s11240-024-02721-9 Aberrant somatic embryo Embryogenic cell line Pinus radiata Somatic embryogenesis Zygotic embryo Biotecnología agraria Química Agricultural biotechnology Chemistry Genetic stability, amino acid, and polyamine profile analyses in radiata pine somatic embryos maturated at high temperatures info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion info:eu-repo/semantics/openAccess Plant Cell, Tissue and Organ Culture (PCTOC) 157 3 THUMBNAIL Montalban-pctoc_2024.pdf.jpg Montalban-pctoc_2024.pdf.jpg IM Thumbnail image/jpeg 4913 https://riubu.ubu.es/bitstream/10259/9806/3/Montalban-pctoc_2024.pdf.jpg a855d86b11cbcfb0913e543fc43ffeb8 MD5 3 LICENSE license.txt license.txt text/plain; charset=utf-8 1362 https://riubu.ubu.es/bitstream/10259/9806/2/license.txt 5d013bfa6e473ff0db22cd82a4d71a70 MD5 2 ORIGINAL Montalban-pctoc_2024.pdf Montalban-pctoc_2024.pdf application/pdf 1487752 https://riubu.ubu.es/bitstream/10259/9806/1/Montalban-pctoc_2024.pdf 794ce23ad743a319c2d831dc9b2d445a MD5 1 10259/9806 oai:riubu.ubu.es:10259/9806 2025-03-19 00:42:10.568 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es RWwgYXV0b3IgY29tbyDDum5pY28gdGl0dWxhciBkZSBsb3MgZGVyZWNob3MgZGUgcHJvcGllZGFkIGludGVsZWN0dWFsIGRlIGxhIG9icmEsIG8gZGlzcG9uaWVuZG8gZGUgbG9zIGRlYmlkb3MgcGVybWlzb3MgZGUgbG9zIG90cm9zIHRpdHVsYXJlcywgc2kgbG9zIGh1YmllcmEsIHkgZW4gdmlydHVkIGRlIGxvcyBkZXJlY2hvcyBxdWUgbGUgY29uZmllcmUgbGEgbGVnaXNsYWNpw7NuIHZpZ2VudGUgc29icmUgcHJvcGllZGFkIGludGVsZWN0dWFsIHkgZGVyZWNob3MgZGUgYXV0b3IsIApBVVRPUklaQSBhIGxhIFVuaXZlcnNpZGFkIGRlIEJ1cmdvcyBhIGRpZnVuZGlyLCBkZSBtYW5lcmEgZ3JhdHVpdGEsIGVsIGNvbnRlbmlkbyBkZSBsb3MgYXJjaGl2b3MgZGlnaXRhbGVzIHF1ZSBjb3JyZXNwb25kZW4gYWwgZG9jdW1lbnRvIGRlc2NyaXRvIGFudGVyaW9ybWVudGUsIGNvbiBjYXLDoWN0ZXIgbm8gZXhjbHVzaXZvIHkgZGUgbWFuZXJhIHDDumJsaWNhIGVuIGFjY2VzbyBhYmllcnRvIGEgdHJhdsOpcyBkZSBJbnRlcm5ldCwgcGFyYSBsbyBxdWUgbGEgQmlibGlvdGVjYSBwcm9jZWRlcsOhIGEgYXJjaGl2YXJsb3MgZW4gZWwgUmVwb3NpdG9yaW8gSW5zdGl0dWNpb25hbC4gQXNpbWlzbW8gYXV0b3JpemEgYSBsYSBVbml2ZXJzaWRhZCBkZSBCdXJnb3MgYSByZWFsaXphciBsYXMgdHJhbnNmb3JtYWNpb25lcyBuZWNlc2FyaWFzIGRlIGZvcm1hdG8sIG5vIGRlIGNvbnRlbmlkbywgcGFyYSBnYXJhbnRpemFyIGxhIHByZXNlcnZhY2nDs24geSBlbCBhY2Nlc28gZW4gZWwgZnV0dXJvLgoKRWwgYXV0b3IgZGlzcG9uZSwgZW4gdG9kbyBjYXNvLCBkZWwgZGVyZWNobyBhIHJldm9jYXIgZXN0YSBhdXRvcml6YWNpw7NuLgoKTGEgY2VzacOzbiBkZSBkZXJlY2hvcyBkZSBlc3RhIG9icmEgc2UgZW5jdWVudHJhIHN1amV0YSBhIGxhIGxlZ2lzbGFjacOzbiB2aWdlbnRlIHNvYnJlIHByb3BpZWRhZCBpbnRlbGVjdHVhbCB5IGRlcmVjaG9zIGRlIGF1dG9yLiBTdSBkaWZ1c2nDs24gZW4gZWwgUmVwb3NpdG9yaW8gc2Vyw6EgYmFqbyBsYSBtb2RhbGlkYWQgZGUgbGljZW5jaWEgQ3JlYXRpdmUgQ29tbW9ucyBvIGVxdWl2YWxlbnRlOiByZWNvbm9jaW1pZW50byDigJMgdXNvIG5vIGNvbWVyY2lhbCDigJMgc2luIG9icmEgZGVyaXZhZGEsIHBvciBsYSBxdWUgc2UgcGVybWl0ZSBoYWNlciBjb3BpYSwgZGlzdHJpYnVpciB5IGNvbXVuaWNhciBww7pibGljYW1lbnRlIGxhIG9icmEgc2llbXByZSBxdWUgc2UgY2l0ZSBhbCBhdXRvciwgZWwgdXNvIHF1ZSBzZSBoYWdhIGRlIGVsbGEgc2VhIG5vIGNvbWVyY2lhbCB5IG5vIHNlIGNyZWVuIG9icmFzIGRlcml2YWRhcyBhIHBhcnRpciBkZSBsYSBvcmlnaW5hbC4K