2026-06-01T04:21:18Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/99242025-01-16T01:05:34Zcom_10259_4862com_10259_5086com_10259_2604col_10259_4863

Herrerías-González, Fernando 315d6146-22f7-4b37-ac5e-5b82edab8ac0 500 Yeramian, Andrée d065959e-e201-42d4-8a10-0e22314e49ce 500 Baena-Fustegueras, Juan Antonio 728e3235-e4bb-44f0-8401-9a329b033903 500 Bueno, Marta d2dde1e9-6eff-43f6-8faa-465a6f1ff8e5 500 Fleitas, Catherine 19e1d3b9-a5e7-4be9-946b-4e6c163bd474 500 de la Fuente, Maricruz 1a51c1c0-5c52-408b-9530-69f57725817e 500 Serrano, José C. E. 40b21ff7-6e86-408e-8549-482191b77df5 500 Granado-Serrano, Ana 86cd86bd-98d8-46a9-a099-db825ffbe3ac 500 Santamaría, Maite 550ff9d8-6934-4890-979f-d1db697f6e82 500 Yeramian Hakim, Nadine 9e6e849e-5f47-4176-b233-9d5cde970263 600 Zorzano-Martínez, Marta e48914f5-2126-4bc5-8323-e40a4e6f6f0a 500 Mora, Conchi 2e14846b-d278-4abd-940b-2f0edfacb814 500 Lecube, Albert 2ddac5e4-43ab-4806-bae8-f2fae56b4986 500 2025-01-15T11:06:37Z 2025-01-15T11:06:37Z 2023-05 http://hdl.handle.net/10259/9924 10.3390/nu15102414 2072-6643 Adipocyte dysfunction is the driver of obesity and correlates with insulin resistance and the onset of type 2 diabetes. Protein kinase N1 (PKN1) is a serine/threonine kinase that has been shown to contribute to Glut4 translocation to the membrane and glucose transport. Here, we evaluated the role of PKN1 in glucose metabolism under insulin-resistant conditions in primary visceral adipose tissue (VAT) from 31 patients with obesity and in murine 3T3-L1 adipocytes. In addition, in vitro studies in human VAT samples and mouse adipocytes were conducted to investigate the role of PKN1 in the adipogenic maturation process and glucose homeostasis control. We show that insulinresistant adipocytes present a decrease in PKN1 activation levels compared to nondiabetic control counterparts. We further show that PKN1 controls the adipogenesis process and glucose metabolism. PKN1-silenced adipocytes present a decrease in both differentiation process and glucose uptake, with a concomitant decrease in the expression levels of adipogenic markers, such as PPARγ, FABP4, adiponectin and CEBPα. Altogether, these results point to PKN1 as a regulator of key signaling pathways involved in adipocyte differentiation and as an emerging player of adipocyte insulin responsiveness. These findings may provide new therapeutic approaches for the management of insulin resistance in type 2 diabetes. This research was funded by Plan Estratégico de Investigación e Innovación en Salud from the Generalitat de Catalunya, grant number PERIS SLT002/16/000276 and the Instituto de Salud Carlos III (PI18/00964), Fondos FEDER “Una manera de hacer Europa”. C.M. has received funding from Ministerio de industria, Economía y competitividad (Ref.SAF2017-82567-R). application/pdf spa MDPI Nutrients. V. 15, n. 10, p. 2414 https://doi.org/10.3390/nu15102414 Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess PKN1 Visceral adipose tissue Insulin resistance Type 2 diabetes Adipocyte Glucose metabolism Salud Metabolismo Health Metabolism PKN1 Kinase: A Key Player in Adipocyte Differentiation and Glucose Metabolism info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Nutrients 15 10 2414 THUMBNAIL Herrerías-nutrients_2023.pdf.jpg Herrerías-nutrients_2023.pdf.jpg IM Thumbnail image/jpeg 3436 https://riubu.ubu.es/bitstream/10259/9924/4/Herrer%c3%adas-nutrients_2023.pdf.jpg 948f92cfb4acb169217d6efdc5b1ebd3 MD5 4 LICENSE license.txt license.txt text/plain; charset=utf-8 999 https://riubu.ubu.es/bitstream/10259/9924/3/license.txt b295bcbce42e2caabeb0c623d3860c06 MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 908 https://riubu.ubu.es/bitstream/10259/9924/2/license_rdf 0175ea4a2d4caec4bbcc37e300941108 MD5 2 ORIGINAL Herrerías-nutrients_2023.pdf Herrerías-nutrients_2023.pdf application/pdf 2844938 https://riubu.ubu.es/bitstream/10259/9924/1/Herrer%c3%adas-nutrients_2023.pdf 65d03ff81c765f616593b254cde912ee MD5 1 10259/9924 oai:riubu.ubu.es:10259/9924 2025-01-16 02:05:34.673 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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