2026-04-22T17:32:05Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/99872025-06-12T11:23:01Zcom_10259_9895com_10259_3989com_10259.4_106com_10259_2604col_10259_9896

00925njm 22002777a 4500 dc Merchán Carrillo, Ana author Pérez Fernández, Cristian Antonio author López, María J. author Moreno Casco, José Joaquín author Moreno-Montoya, Margarita author Sánchez Santed, Fernando author Flores, Pilar author 2021-05 Rationale: Compulsive behaviour, present in different psychiatric disorders such as obsessive-compulsive disorder, schizophrenia and drug abuse, is associated with altered levels of serotonin (5-hydroxytryptamine, 5-HT). The gut microbiota regulates tryptophan (TRP) metabolism and may affect global 5-H synthesis in the enteric and central nervous systems, suggesting a possible involvement of gut microbiota in compulsive spectrum disorders. Objectives: The present study investigated whether chronic TRP depletion by diet alters the faecal bacterial community profiles of compulsive versus non-compulsive rats in schedule-induced polydipsia (SIP). Peripheral plasma 5-HT and brain-derived neurotrophic factor (BDNF) levels were evaluated. Methods: Wistar rats were selected as High Drinkers (HD) or Low Drinkers (LD) according to their SIP behaviour and were fed for 14 days with either a TRP-free diet (T-) or a TRP-supplemented diet (T+). The faecal bacterial community structure was investigated with 16S rRNA gene-targeted denaturing gradient gel electrophoresis (DGGE) fingerprinting analysis. Results: Compulsive HD rats showed a lower bacterial diversity than LD rats, irrespectively of the diet. The TRP-depleted HD rats, the only group increasing compulsive licking in SIP, showed a reduction of bacterial evenness and a highly functionally organized community compared with the other groups, indicating that this bacterial community is more fragile to external changes due to the dominance of a low number of species. The chronic TRP depletion by diet effectively reduced peripheral plasma 5-HT levels in both HD and LD rats, while plasma BDNF levels were not altered. Conclusions: These results highlight the possible implication of reduced microbial diversity in compulsive behaviour and the involvement of the serotonergic system in modulating the gut brain-axis in compulsive spectrum disorders. 0031-9384 http://hdl.handle.net/10259/9987 10.1016/j.physbeh.2021.113356 Compulsivity Schedule-induced polydipsia Gut microbiota Chronic tryptophan depletion PCR-DGGE Brain-derived neurotrophic factor Dietary tryptophan depletion alters the faecal bacterial community structure of compulsive drinker rats in schedule-induced polydipsia