2026-04-22T15:58:37Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/99872025-06-12T11:23:01Zcom_10259_9895com_10259_3989com_10259.4_106com_10259_2604col_10259_9896

Merchán Carrillo, Ana 13d41504-364f-494a-ae73-a6d8c239b2ae 600 Pérez Fernández, Cristian Antonio 934 500 0000-0001-6675-2086 López, María J. abdd75f0-278c-4e06-80e0-e56a6dadf489 Moreno Casco, José Joaquín afef9685-4448-4918-93e5-e0b501dd35a1 Moreno-Montoya, Margarita 8a1a3603-f24f-4d81-845b-1555ef0da966 Sánchez Santed, Fernando 3945434c-d67b-4d35-8c6d-262ce027f039 600 Flores, Pilar d476fe64-9140-498c-8f24-ecb50f9010db 2025-01-22T09:07:54Z 2025-01-22T09:07:54Z 2021-05 0031-9384 http://hdl.handle.net/10259/9987 10.1016/j.physbeh.2021.113356 Rationale: Compulsive behaviour, present in different psychiatric disorders such as obsessive-compulsive disorder, schizophrenia and drug abuse, is associated with altered levels of serotonin (5-hydroxytryptamine, 5-HT). The gut microbiota regulates tryptophan (TRP) metabolism and may affect global 5-H synthesis in the enteric and central nervous systems, suggesting a possible involvement of gut microbiota in compulsive spectrum disorders. Objectives: The present study investigated whether chronic TRP depletion by diet alters the faecal bacterial community profiles of compulsive versus non-compulsive rats in schedule-induced polydipsia (SIP). Peripheral plasma 5-HT and brain-derived neurotrophic factor (BDNF) levels were evaluated. Methods: Wistar rats were selected as High Drinkers (HD) or Low Drinkers (LD) according to their SIP behaviour and were fed for 14 days with either a TRP-free diet (T-) or a TRP-supplemented diet (T+). The faecal bacterial community structure was investigated with 16S rRNA gene-targeted denaturing gradient gel electrophoresis (DGGE) fingerprinting analysis. Results: Compulsive HD rats showed a lower bacterial diversity than LD rats, irrespectively of the diet. The TRP-depleted HD rats, the only group increasing compulsive licking in SIP, showed a reduction of bacterial evenness and a highly functionally organized community compared with the other groups, indicating that this bacterial community is more fragile to external changes due to the dominance of a low number of species. The chronic TRP depletion by diet effectively reduced peripheral plasma 5-HT levels in both HD and LD rats, while plasma BDNF levels were not altered. Conclusions: These results highlight the possible implication of reduced microbial diversity in compulsive behaviour and the involvement of the serotonergic system in modulating the gut brain-axis in compulsive spectrum disorders. This study was funded by grants from the Ministerio de Ciencia, Innovación y Universidades (Spanish Government) and Fondo Europeo de Desarrollo Regional, PSI2017–86,847-C2–1-R and PGC2018–099,117-B-C21, and by University of Almería (PPUENTE2020/003), with the help of the Post-doctoral fellowship “Contrato Puente” at University of Almería. application/pdf eng Elsevier Physiology & Behavior. 2021, V. 233, 113356 https://doi.org/10.1016/j.physbeh.2021.113356 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/PSI2017-86847-C2-1-R/ES/CORRELATOS NEURO-BIOLOGICOS DE LOS DESORDENES DEL NEURODESARROLLO INDUCIDOS POR FACTORES GENETICOS O AMBIENTALES/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-099117-B-C21/ES/HACIA UNA NUEVA CARACTERIZACION Y TRATAMIENTO DE LOS TRASTORNOS DEL ESPECTRO IMPULSIVO-COMPULSIVO: UN ENFOQUE TRASLACIONAL/ info:eu-repo/grantAgreement/UAL//PPUENTE2020%2F003/ Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Compulsivity Schedule-induced polydipsia Gut microbiota Chronic tryptophan depletion PCR-DGGE Brain-derived neurotrophic factor Triptófano Bacterias-Metabolismo Psicología Tryptophan Microbial metabolism Psychology Dietary tryptophan depletion alters the faecal bacterial community structure of compulsive drinker rats in schedule-induced polydipsia info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Physiology & Behavior 233 113356 THUMBNAIL Merchan-pb_2021.pdf.jpg Merchan-pb_2021.pdf.jpg IM Thumbnail image/jpeg 4044 https://riubu.ubu.es/bitstream/10259/9987/4/Merchan-pb_2021.pdf.jpg b124e45288a9edbc6ef8fc10cd52603d MD5 4 LICENSE license.txt license.txt text/plain; charset=utf-8 999 https://riubu.ubu.es/bitstream/10259/9987/3/license.txt b295bcbce42e2caabeb0c623d3860c06 MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 805 https://riubu.ubu.es/bitstream/10259/9987/2/license_rdf 4460e5956bc1d1639be9ae6146a50347 MD5 2 ORIGINAL Merchan-pb_2021.pdf Merchan-pb_2021.pdf application/pdf 664497 https://riubu.ubu.es/bitstream/10259/9987/1/Merchan-pb_2021.pdf a99fef7c21b7f65e0eda2bdaaddfe2d8 MD5 1 10259/9987 oai:riubu.ubu.es:10259/9987 2025-06-12 13:23:01.382 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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