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<mods:namePart>Yeramian Hakim, Nadine</mods:namePart>
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<mods:namePart>Chaya, C.</mods:namePart>
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<mods:namePart>Suárez Lepe, J. A.</mods:namePart>
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<mods:abstract>In an attempt to increase the acidity of wine by biological means, malate-producing yeasts were selected from a collection of 282 strains isolated in different parts of Spain. Only 4% of these strains (all of which belonged to Saccharomyces cerevisiae) produced l-(−)-malic acid in the range of 0.5−1 g/L. This was formed between days 2 and 6 of alcoholic fermentation, reaching a maximum on days 3 and 4; the concentration remained stable from day 7. Malic acid production was favored by temperatures in the 18−25 °C range and by musts with a high pH and low concentrations of sugar, initial malic acid, and yeast-assimilable nitrogen. Oxaloacetic acid, a precursor of malic acid, had no influence on malate production. The precursors pyruvic and fumaric acid did, however, have a significant effect on the production of this acid in some strains. No direct relation between pyruvate and malate metabolism was observed.</mods:abstract>
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<mods:topic>Wine acidity</mods:topic>
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<mods:topic>Biological acidification</mods:topic>
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<mods:topic>Malic acid</mods:topic>
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<mods:topic>Alcoholic fermentation</mods:topic>
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<mods:topic>Saccharomyces cerevisiae</mods:topic>
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<mods:topic>Precursors</mods:topic>
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<mods:topic>Physicochemical variables</mods:topic>
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<mods:title>l-(−)-Malic Acid Production bySaccharomycesspp. during the Alcoholic Fermentation of Wine (1)</mods:title>
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