RT info:eu-repo/semantics/article
T1 Membrane fractionation of hydrolysates of the water-soluble protein from tuna fish meal obtained by subcritical water and enzymatic treatments. Comparison of physical and chemical properties
A1 Barea Gómez, Pedro
A1 Illera Gigante, Alba Ester
A1 Candela Gil, Helena
A1 Melgosa Gómez, Rodrigo
A1 Benito Moreno, José Manuel
A1 Beltrán Calvo, Sagrario
A1 Sanz Díez, Mª Teresa
K1 Protein hydrolysis
K1 Dead-end fltration (UF and NF)
K1 Protein and Free amino acids profle
K1 Reducing and chelating capacities
K1 Molecular weight distribution
K1 Ingeniería Química
K1 Chemical engineering
K1 Biotecnología
K1 Biotechnology
K1 Bioquímica
K1 Biochemistry
AB Two different hydrolysates of the water-soluble protein (WSP) fraction from tuna fish meal were obtained by subcritical water-CO2 (subW-CO2) and Alcalase® treatments. Hydrolysates showed different chemical composition regarding their free amino acid (FAA) profile and molecular weight distribution of the peptides generated. Consequently, different strategies were proposed by using a stirred dead-end filtration system equipped with flat sheet membranes. Two nanofiltration (NF) consecutive steps were proposed for fractionation of subW-CO2 hydrolysates with polyamide membranes (800–600 Da and 300–150 Da) yielding a first retentate where 79% of the protein fraction was retained, while 92% of FAA were retained in the second step with a purity index of 29%. Alcalase® hydrolysates were fractionated by ultrafiltration (UF, 10 kDa) followed by NF (1–1.1 kDa) with polyethersulfone membranes obtaining a first retentate with more than 65% of protein fraction retention with a purity index of 50%. A relatively wide molecular weight distribution was observed in all streams, with high antioxidant and iron-chelating capacities in the retentate fractions.
PB Springer
YR 2025
FD 2025-03
LK http://hdl.handle.net/10259/10443
UL http://hdl.handle.net/10259/10443
LA eng
NO This work was supported by the Agencia Estatal de Investigación [Grant numbers PID2022-136385OB-I00, PDC2022-133443-I00] and the Junta de Castilla y León (JCyL) and the European Regional Development Fund (ERDF) [Grant number BU027P23]. P. Barea pre-doctoral contract was funded by JCyL and the European Social Fund (ESF) [ORDEN EDU/1868/2022]. A. E. Illera postdoctoral contract was funded by BU027P23. H. Candela-Gil contract was funded by PDC2022-133443-I00. R. Melgosa contract was funded by a Beatriz Galindo Research Fellowship [BG20/00182].
DS Repositorio Institucional de la Universidad de Burgos
RD 11-may-2025