RT info:eu-repo/semantics/article T1 Fast-track analysis of beetroot using the liquid generated during the cooking process A1 Gaona Ruiz, María A1 Vallejos Calzada, Saúl A1 Rodríguez Velasco, Ana A1 Trigo López, Miriam A1 Carrillo Pérez, Celia K1 Ready-to-eat cooked beetroot K1 Beetroot liquid K1 Betalains K1 Polyphenols K1 Total antioxidant capacity K1 Alimentos-Composición K1 Food-Composition K1 Alimentos-Análisis K1 Food-Analysis AB Beetroots are vegetables with high antioxidant capacity owing to their content of bioactive compounds such as betalains, polyphenols, and other antioxidants, which serve as quality markers for beetroot products. Conventional methods for determining these compounds rely on time-consuming solid-liquid extractions requiring large amounts of solvents, which is a drawback in the food industry. This study, the first to evaluate the usefulness of the liquid present in ready-to-eat cooked beetroots for functional characterisation, aimed to develop a faster, more sustainable alternative in collaboration with the beetroot industry. Beetroot liquid and extracts (from beetroot flesh after conventional extraction) from 44 beetroot samples were analysed for betalain and polyphenol content and profile, and total antioxidant capacity. Results showed that beetroot liquid reliably indicated changes in bioactive compounds and bioactivity under increasing intensities of thermal treatment, with an identical betalain and polyphenol profile to that obtained via mass spectrometry in extracts. A linear regression model enabled the prediction of beetroot functionality, reducing pre-quantification time from 105 min to 5 min and eliminating the need for 10 mL of solvent per gram of sample. Validation with commercial ready-to-eat beetroots confirmed the method's reliability. This novel approach offers the beetroot industry a rapid, capable of being automated, and more sustainable alternative for quality assessment, ensuring efficient monitoring of beetroot functionality. PB Elsevier SN 2772-5022 YR 2025 FD 2025-06 LK https://hdl.handle.net/10259/11217 UL https://hdl.handle.net/10259/11217 LA eng NO The financial support provided by Fondo Europeo de Desarrollo Regional-European Regional Development Fund (FEDER, ERDF) and Regional Government of Castilla y León -Consejería de Educación, Junta de Castilla y León- (BU025P23) is gratefully acknowledged. This work was supported by the Regional Government of Castilla y León (Junta de Castilla y León) and by the Ministry of Science and Innovation MICIN and the European Union NextGenerationEU PRTR. Author Saul Vallejos received grant BG22/00086 funded by Spanish Ministerio de Universidades. Author María Gaona-Ruiz received a research assistant contract (UBU-08-B) funded by the Regional Government of Castilla y León. We also acknowledge the financial support provided by MCIN/AEI/ 10.13039/501100011033 and by “ERDF A way of making Europe” (grant PID2023–147301OB-I00). DS Repositorio Institucional de la Universidad de Burgos RD 28-abr-2026