RT info:eu-repo/semantics/article
T1 Evaluation of factors influencing the growth of non-toxigenic Clostridium botulinum type E and Clostridium sp. in high-pressure processed and conditioned tender coconut water from Thailand
A1 González Angulo, Mario
A1 Clauwers, Charlien
A1 Harastani, Rania
A1 Tonello Samson, Carole
A1 Jaime Moreno, Isabel
A1 Rovira Carballido, Jordi
A1 W. Michiels, Chris
K1 High pressure processing
K1 Coconut water
K1 Clostridium botulinum
K1 Dissolved oxygen
K1 Germinant
K1 Amino acid
K1 Nutrients
K1 Microbiología
K1 Microbiology
K1 Microbiología alimentaria
K1 Food-Microbiology
AB Bacterial spores survive high pressure processing (HPP). Group II Clostridium botulinum is an obligate anaerobe spore-forming pathogen that can produce the botulinum neurotoxin under refrigeration. This study assessed nontoxigenic type E C. botulinum and Group II Clostridium sp. growth in raw and HPP (550 MPa, 3 min, 10 °C) Thai coconut water (CCW; pH 5.2). No spore germination or growth occurred in HPP CCW inoculated with 105 CFU/ml after 61 days regardless of oxygen concentration (<0.5 – 11 mg/l) or storage temperature (4 and 20 °C). Spore concentration decreased by 3.0 ± 0.1 log CFU/ml in a worst-case scenario consisting of non-HPP filter-sterilized CCW (pH 7.0) under anoxic incubation at 30 °C during 61 days, suggesting spore germination followed by cellular death. Supplementing filter-sterilized CCW (pH 7.0) with selected germinants and free amino acids did not support spore development, but the addition of nutrient-rich laboratory media (TPGY broth) at low concentrations (6.25%) promoted growth, suggesting that a lack of nutrients prevents C. botulinum development in CCW. Further risk assessment will require evaluating other CCW varieties and toxin production.
PB Elsevier
SN 0963-9969
YR 2020
FD 2020-04
LK https://hdl.handle.net/10259/11244
UL https://hdl.handle.net/10259/11244
LA eng
DS Repositorio Institucional de la Universidad de Burgos
RD 28-abr-2026