RT info:eu-repo/semantics/article
T1 Identification of PimR as a Positive Regulator of Pimaricin Biosynthesis in            Streptomyces natalensis
A1 Antón Fidalgo, Nuria
A1 Mendes, Marta V.
A1 Martín, Juan F.
A1 Aparicio, Jesús F.
K1 Microbiología
K1 Microbiology
K1 Bioquímica
K1 Biochemistry
K1 Biología molecular
K1 Molecular biology
K1 Genética
K1 Genetics
AB Sequencing of the DNA region on the left fringe of the pimaricin gene cluster revealed the presence of a 3.6-kb gene, pimR, whose deduced product (1,198 amino acid residues) was found to have amino acid sequence homology with bacterial regulatory proteins. Database comparisons revealed that PimR represents the archetype of a new class of regulators, combining a Streptomyces antibiotic regulatory protein (SARP)-like N-terminal section with a C-terminal half homologous to guanylate cyclases and large ATP-binding regulators of the LuxR family. Gene replacement of pimR from Streptomyces natalensis chromosome results in a complete loss of pimaricin production, suggesting that PimR is a positive regulator of pimaricin biosynthesis. Gene expression analysis by reverse transcriptase PCR (RT-PCR) of the pimaricin gene cluster revealed that S. natalensis ΔPimR shows no expression at all of the cholesterol oxidase-encoding gene pimE, and very low level transcription of the remaining genes of the cluster except for the mutant pimR gene, thus demonstrating that this regulator activates the transcription of all the genes belonging to the pimaricin gene cluster but not its own transcription.
PB American Society for Microbiology
SN 0021-9193
YR 2004
FD 2004-05
LK https://hdl.handle.net/10259/11321
UL https://hdl.handle.net/10259/11321
LA eng
NO This work was supported by a grant from the Comisio´n Interministerial de Ciencia y Tecnología (CICYT) to J.F.A. (BIO2001--0040). M.V.M. received a fellowship of the Fundac¸˜ao para a Cieˆncia e a Tecnologia (PRAXIS XXI/BD/15850/98). N.A. was the recipient of an F.P.U. fellowship from Ministerio de Educacio´n, Cultura, y Deporte (AP2002-1446).
DS Repositorio Institucional de la Universidad de Burgos
RD 24-may-2026