ANEXO I This readme.txt file was generated on 2024-05-30 by Aranzazu Heras Vidaurre GENERAL INFORMATION 1. TITLE OF DATASET: Dataset of the work “UV Vis absorption spectroelectrochemistry of folic acid” 2. AUTHORSHIP: Name: Fabiola Olmo-Alonso Institution: Departamento de Quimica. Universidad de Burgos e-mail: folmo@ubu.es ORCID: 0000-0002-4069-3599 Name: Andrea Rodriguez Institution: Departamento de Quimica. Universidad de Burgos e-mail: andrearr@ubu.es Name: Alvaro Colina Institution: Departamento de Quimica. Universidad de Burgos e-mail: acolina@ubu.es ORCID: 0000-0003-0339-356X Name: Aranzazu Heras Institution: Departamento de Quimica. Universidad de Burgos e-mail: maheras@ubu.es ORCID: 0000-0002-5068-2164 DESCRIPTION 1. DATASET LANGUAGE: English 2. KEYWORDS: Vitamin B9, Pharmaceutical drug, Electrochemistry, UV/Vis absorption spectroscopy 3. ABSTRACT: UV/Vis absorption spectroelectrochemistry is a very promising analytical technique due to the complementary information that is simultaneously obtained from electrochemistry and spectroscopy. In this work, this technique is used in a parallel configuration to study the oxidation of folic acid in alkaline medium. Herein, UV/Vis absorption spectroelectrochemistry has been used to detect both the oxidation products and the folic acid consumed at the electrode/solution interface, allowing us to develop an analytical protocol to quantify vitamin B9 in pharmaceutical tablets. Linear ranges of three orders of magnitude have been achieved in basic medium (pH = 12.9), obtaining high repeatability and low detection limits. The spectroelectrochemical determination of folic acid in pharmaceutical tablets at alkaline pH values is particularly interesting because of the changes that occur in the optical signal during the electrochemical oxidation of FA, providing results with very good figures of merit and demonstrating the utility and versatility of this hyphenated technique, UV/Vis absorption spectroelectrochemistry. 4. DATE OF DATA COLLECTION: 2019 5. DATE OF DATASET PUBLICATION: 30-05-2024 6. FUNDING: Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. Authors acknowledge the financial support from Ministerio de Economía y Competitividad (Grant CTQ2017-83935-R-AEI/FEDERUE), Junta de Castilla y León (Grant BU297P18), and Ministerio de Ciencia, Innovación y Universidades (Grant RED2018-102412-T). F.O. is grateful for the contract funded by Junta de Castilla y León, the European Social Fund, and the Youth Employment Initiative. ACCESS INFORMATION 1. LICENSE: This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/) 2. DOI: https://doi.org/10.1007/s10008-021-05026-5 3. RELATED PUBLICATIONS: Article published in Journal of Solid State Electrochemistry, 26 (2022) 29-37 (Springer Nature) METHODOLOGICAL INFORMATION UV/Vis-SEC measurements were performed using a customized UV–VIS SPELEC instrument (Metrohm-DropSens), employing the experimental setup described previously. A deuterium lamp was used because the reagents and products of this electrochemical process absorb electromagnetic radiation in the UV region. Carbon DRP-110 screen-printed electrodes (SPE, Metrohm-DropSens) were used for UV/Vis-SEC measurements. These C-SPEs consist of a carbon working electrode (C-WE) of 4 mm diameter, a silver pseudo-reference electrode, and a carbon counter electrode. UV/Vis-SEC experiments in normal configuration were carried out using the Teflon® SEC cell for reflection experiments (DRP-REFLECELL, Metrohm-DropSens) and a reflection probe (DRP-RPROBE, Metrohm-DropSens). UV/Vis-SEC experiments in parallel configuration were carried out by facing and attaching two 100-?m-bare optical fibers (Avantes) to the surface of the C-WE of the SPEs that were placed at the boxed connector for SPEs (DSC, Metrohm-DropSens). The optical pathway was measured for each C-SPE used, which is in all cases between 2 and 3 mm in length. A 50-?L aliquot of the solution studied is used in each experiment. Matlab R2018a is the software used for the treatment and analysis of the data generated. FILE OVERVIEW E01_CV_buffer_FA_0.1mM_10mV_0.7-1.3V_pH5.csv E01_CV_KCl_FA_001M_10mV_0.7-1.3V_pH7.csv E01_CV_NaOH_FA_01mM_20mV_0_09_-1V_pH13.csv E02_CV_P_FA_01mM_20mV_0_09_-1V.csv E02_CV_P_NaOH_01M_20mV_0_09_-1V.csv E01_CV_Blanco1_NaOH_01M_20mV_0_09_R1.csv E01_CV_Blanco1_NaOH_01M_20mV_0_09_R2.csv E01_CV_Blanco1_NaOH_01M_20mV_0_09_R3.csv E01_CV_FA_001mM_20mV_0_09_R1.csv E01_CV_FA_001mM_20mV_0_09_R2.csv E01_CV_FA_001mM_20mV_0_09_R3.csv E01_CV_FA_01mM_20mV_0_09_R1.csv E01_CV_FA_01mM_20mV_0_09_R2.csv E01_CV_FA_01mM_20mV_0_09_R3.csv E01_CV_FA_002mM_20mV_0_09_R1.csv E01_CV_FA_002mM_20mV_0_09_R2.csv E01_CV_FA_002mM_20mV_0_09_R3.csv E01_CV_FA_004mM_20mV_0_09_R1.csv E01_CV_FA_004mM_20mV_0_09_R2.csv E01_CV_FA_004mM_20mV_0_09_R3.csv E01_CV_FA_0005mM_20mV_0_09_R1.csv E01_CV_FA_0005mM_20mV_0_09_R2.csv E01_CV_FA_0005mM_20mV_0_09_R3.csv E01_CV_FA_006mM_20mV_0_09_R1.csv E01_CV_FA_006mM_20mV_0_09_R2.csv E01_CV_FA_006mM_20mV_0_09_R3.csv E01_CV_FA_0008mM_20mV_0_09_R1.csv E01_CV_FA_0008mM_20mV_0_09_R2.csv E01_CV_FA_0008mM_20mV_0_09_R3.csv E01_CV_FA_008mM_20mV_0_09_R1.csv E01_CV_FA_008mM_20mV_0_09_R2.csv E01_CV_FA_008mM_20mV_0_09_R3.csv E01_CV_Blanco1_NaOH_01M_20mV_0_09_R1.csv E01_CV_Blanco2_NaOH_01M_20mV_0_09_R1.csv E01_CV_FA_001mM_20mV_0_09_R1.csv E01_CV_FA_01mM_20mV_0_09_R1.csv E01_CV_FA_01mM_20mV_0_09_R1_Réplica.csv E01_CV_FA_002mM_20mV_0_09_R1.csv E01_CV_FA_004mM_20mV_0_09_R1.csv E01_CV_FA_004mM_20mV_0_09_R1_Réplica.csv E01_CV_FA_0005mM_20mV_0_09_R1.csv E01_CV_FA_0005mM_20mV_0_09_R1_Réplica.csv E01_CV_FA_006mM_20mV_0_09_R1.csv E01_CV_FA_006mM_20mV_0_09_R1_Réplica.csv E01_CV_FA_0008mM_20mV_0_09_R1.csv E01_CV_FA_008mM_20mV_0_09_R1.csv E01_CV_FA_P1_20mV_0_09_R1.csv E01_CV_FA_P2_20mV_0_09_R1.csv E01_CV_FA_P3_20mV_0_09_R1.csv DATA-SPECIFIC INFORMATION Each “.csv” experiment includes a file with a matrix that include information about time, potential, current, wavelength and intensity.