ANEXO I: This readme.txt file was generated on 2022-06-23 by Saul Vallejos Calzada GENERAL INFORMATION 1. Title of dataset: Dataset of the work "STRAIGHTFORWARD PURIFICATION METHOD FOR THE DETERMINATION OF THE ACTIVITY OF GLUCOSE OXIDASE AND CATALASE IN HONEY BY EXTRACTING POLYPHENOLS WITH A FILM-SHAPED POLYMER" 2. Autorship Name: Lara Gonzalez Ceballos Institution: Departamento de Biotecnología y Ciencia de los Alimentos. Universidad de Burgos Email: lgceballos@ubu.es ORCID: https://orcid.org/0000-0002-8983-7362 Name: Jose Carlos Guirado Moreno Institution: Departamento de Química. Universidad de Burgos Email: jcguirado@ubu.es ORCID: https://orcid.org/0000-0001-6233-5496 Name: Gianluca Utzeri Institution: Departamento de Quimica. Universidade de Coimbra Email: gianlucautz@gmail.com ORCID: https://orcid.org/0000-0002-8457-4217 Name: José Miguel García Pérez Institution: Departamento de Química. Universidad de Burgos Email: svallejos@ubu.es ORCID: https://orcid.org/0000-0002-2674-8194 Name: Miguel Angel Fernandez Muiño Institution: Departamento de Biotecnología y Ciencia de los Alimentos. Universidad de Burgos Email: mafernan@ubu.es ORCID: https://orcid.org/0000-0001-5982-2293 Name: Sandra María Osés Gómez Institution: Departamento de Biotecnología y Ciencia de los Alimentos. Universidad de Burgos Email: smoses@ubu.es ORCID: https://orcid.org/0000-0003-2854-1346 Name: Maria Teresa Sancho Institution: Departamento de Biotecnología y Ciencia de los AlimentosUniversidad de Burgos Email: mtsancho@ubu.es ORCID: https://orcid.org/0000-0002-9128-9422 Name: Ana Arnaiz Alonso Institution: Departamento de Química. Universidad de Burgos Email: anaaa@ubu.es ORCID: https://orcid.org/0000-0002-3842-498X Name: Artur JM Valente Institution: Departamento de Quimica. Universidade de Coimbra Email: avalente@ci.uc.pt ORCID: https://orcid.org/0000-0002-4612-7686 Name: Saul Vallejos Calzada Institution: Departamento de Química. Universidad de Burgos Email: svallejos@ubu.es ORCID: https://orcid.org/0000-0001-5522-6574 DESCRIPTION ----------- 1. Dataset language English 2. Abstract: The dataset contains all raw data of the work "STRAIGHTFORWARD PURIFICATION METHOD FOR THE DETERMINATION OF THE ACTIVITY OF GLUCOSE OXIDASE AND CATALASE IN HONEY BY EXTRACTING POLYPHENOLS WITH A FILM-SHAPED POLYMER" 3. Keywords: Catechol, 1,2-dihydroxyphenol, phenylboronic acid, glucose oxidase (GOX), catalase (CAT), honey 4. Date of data collection 2021-2022 5. Date of dataset publication 2022-06-23 6. Funding We gratefully acknowledge the financial support provided by all funders. Author Saul Vallejos received funding from "La Caixa" Foundation Grant LCF/PR/PR18/51130007. Author Jose Miguel García received funding from “Spanish Agencia Estatal de Investigación " Grant PID2020-113264RB-I00 / AEI / 10.13039/501100011033. Ana Arnaiz received funding from Ministerio de Universidades-European Union in the frame of NextGenerationEU RD 289/2021 (Universidad Politécnica de Madrid). We also gratefully acknowledge European Regional Development Fund (ERDF). Gianluca Utzeri thanks Fundação para a Ciência e a Tecnologia (FCT, Portugal) for PhD grant (SFR/BD/146358/2019). The Coimbra Chemistry Centre is supported by the FCT, through Projects UIDB/00313/2020 and UIDP/00313/2020. 7. Geographic location/s of data collection Burgos (Spain), Coimbra (Portugal) ACCESS INFORMATION ------------------ 1. Dataset Creative Commons License: CC 4.0 Internacional 2. Dataset DOI: 10.36443/10259/6739 (http://doi.org/10.36443/10259/6739) 3. Related publication The related article has been sent to: "Food Chemistry" ISSN:0308-8146 METHODOLOGICAL INFORMATION -------------------------- Inductively coupled plasma mass spectrometry (ICP‒MS) measurements were recorded on an Agilent 7500 ICP‒MS spectrometer (Agilent, Santa Clara, USA). 1H and 13C{1H} NMR spectra (Avance III HD spectrometer, Bruker Corporation, Billerica, Massachusetts, USA) were recorded at 300 MHz for 1H and 75 MHz for 13C using deuterated solvents such as dimethyl sulfoxide (DMSO‒d6) at 25 °C. The weight percentage of water taken up by the films upon soaking in pure water at 20°C until reaching equilibrium (water‒swelling percentage, WSP) was obtained from the weight of a dry sample film (ωd) and its water‒swelled weight (ωs) using the following expression: WSP=100×[(ωs−ωd)/ωd]. The powder X‒ray diffraction (PXRD) patterns were obtained using a diffractometer (D8 Discover Davinci design, Bruker Corporation, Billerica, Massachusetts, USA) operating at 40 kV, using Cu(Kα) as the radiation source, a scan step size of 0.02°, and a scan step time of 2 s. The polymers were thermally characterized by using thermogravimetric analysis (Q50 TGA analyser, TA Instruments, New Castle, DE, USA) with 10–15 mg of sample under synthetic air and nitrogen atmosphere at 10°C·min−1; differential scanning calorimetry, with 10–15 mg of the sample under a nitrogen atmosphere at a heating rate of 10°C min−1 (Q200 DSC analyser, TA Instruments, New Castle, DE, USA); and tensile properties analysis, with 5 × 9.44 × 0.103 mm samples tested at 5 mm min−1 (EZ Test Compact Table-Top Universal Tester, Shimadzu Kyoto, Japan). Infrared spectra (FTIR) were recorded with an infrared spectrometer (FT/IR‒4200, Jasco, Tokyo, Japan) with an ATR‒PRO410‒S single reflection accessory. Isothermal titration calorimetry (ITC) measurements were performed using a microcalorimeter (VP‒ITC MicroCal Inc., Malvern, UK) equipped with two cells, one cell for sample (Linear‒bor), at a final concentration of phenylboronic acid groups of 0.61 mM, and another one for reference, with volumes of 1.436 mL. The titrant syringe was filled with 280 µL solution (9 M) of the tested compounds (catechol, resorcinol, hydroquinone, fructose, glucose, and maltose), which was step-by-step added into the sample cell (20 L aliquots were added every 5 min). All the solutions were degassed for 10 min at 25.0 ± 0.1°C in a vacuum pump to avoid blistering in the syringe or in the calorimetric cells during the experiment. Calorimetric measurements were performed at 25.000 ± 0.001°C, with a constant stirring at 307 rpm in the sample cell. The catechol concentration in aqueous solutions used in the permeation, kinetic and sorption isotherm analysis was determined via UV‒Vis spectrophotometer measurements (UV‒2600i, Shimadzu, Germany). Calibration line was performed for catechol in the analytical range 0‒50 mg L−1 by using the absorbance at λmax = 275 nm; a molar absorption coefficient equal to xxxx L mol−1 cm−1 was obtained. The analysis was performed in duplicate, and the solutions were prepared in milli-Q ultrapure water. Enzyme interferents assays were performed using a Synergy HT microplate reader (BioTek®, Winooski, Vermont, USA) measuring absorbance at 452 nm. Concentration of catalase, glucose‒oxidase, H2O2, and the compounds used as interferents was determined using a Hitachi U‒3900 UV‒Vis spectrophotometer (Tokyo, Japan). The enzymatic (GOX and CAT) activities were measured using a CARY 400 Bio UV‒Visible Spectrophotometer, measuring the absorbance, for both analyses, at 400 nm. FILE OVERVIEW -------------- H202 Standard Curve.ods H202 Standard.ods 2way ANOVA of CAT inh Grouped Data.txt CAT inh DATA.txt CAT inh GROUPED DATA.txt Normality and Lognormality Tests of CAT inh DATA.txt Tukey's test of CAT inh Grouped Data.txt Data and analyses CAT inhibition with all compounds.ods CAT INH 10 MIN_Data replicate 1.ods CAT INH 10 MIN_Data replicate 2.ods CAT INH 10 MIN_Data replicate 3.ods Normality and Lognormality Tests of GOX inh NORMALITY DATA.txt Tukey's test of GOX inh Grouped data.txt 2way ANOVA of GOX inh Grouped data.txt GOX inh Grouped data.txt GOX inh NORMALITY DATA.txt Data and analyses GOX inhibition with all compounds.ods GOX INH 1H_Data replicate 1.ods GOX INH 1H_Data replicate 2.ods GOX INH 1H_Data replicate 3.ods ITC.ods kinetic-CAT out of cuvette 2.ods kinetic-CAT out of cuvette 3.ods kinetic-CAT.ods kinetic-RES out of cuvette 1.ods kinetic-RES out of cuvette 2.ods Isotherm-CATECHOL.ods Proof of concept.ods 2way ANOVA of GOX three data.txt 2way ANOVA of GOX two data.txt CATALASE DATA.txt Catalase three data.txt Catalase two data.txt GOX DATA.txt GOX sample 4.txt GOX three data.txt GOX two data.txt Normality and Lognormality Tests of CATALASE DATA.txt Normality and Lognormality Tests of GOX DATA.txt Uncorrected Fisher's LSD Catalase three data.txt Uncorrected Fisher's LSD Catalase two data.txt Uncorrected Fisher's LSD GOX three data.txt Uncorrected Fisher's LSD GOX two data.txt Welch's t test of GOX sample 4.txt 2way ANOVA of Catalase three data.txt 2way ANOVA of Catalase two data.txt UV-Vis.ods FT-IR-Film-bor.txt FT-IR-Linear-bor.txt Linear-bor-13C.csv Linear-bor-1H.csv TGA-Linear-Bor.txt TGA-Film-bor.txt DSC-Linear-Bor.txt DSC-Film-bor.txt PXRD-Linear-bor.xy PXRD-Film-bor.xy ICP-MS.ods WATER_SWEL_PERC_R3.txt WATER_SWEL_PERC_R1.txt WATER_SWEL_PERC_R2.txt Tensile properties.csv Short description: Each file is a .ods, .txt, .xy or .csv file of each study/experiment